Mikrobiol. Z. 2017; 79(3):14-26. Russian.
doi: https://doi.org/10.15407/microbiolj79.03.014

Modification Bacillus thuringiensis var. israelensis IMV В-7465 Peptidase Activity
by Hydrazide/Hydrozone Complexes of 3d-Metals

Varbanets L.D.1, Nidialkova N.А.1, Seifullina I.I.2, Pulia A.V.2, Skorokhod L.S.2

1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

2Odessa National Mechnykov University
2 Dvoryanska Str., Odessa, 65082, Ukraine

Goal. To study the infuence on the activity of Bacillus thuringiensis var. israelensis IMV B-7465 peptidase 1 and 2 peptidase of 15 biocomplexes ions 3d-metal (Co2+, Ni2+, Mn2+, Cu2+, Zn2+) with “gidazepam”-2-(7-Bromo-2-oxo-5-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-1-yl)acetohydrazide (Hydr) and condensation products thereof with pyruvic acid (HPv) and salicylic aldehyde (HSal). According to the type its can be divided into three groups: mixed-ligand molecular, mixed-ligand inner complexes, ligand identical inner complexes. Methods. We used methods of determining elastase, collagenase and fbrinolytic activity. Results. Analysis of the data showed that a signifcant number of the investigated complexes regardless of the concentration of virtually no effect on elastase and collagenase activity B. thuringiensis var. israelensis IMV B-7465 of  peptidase 1. Some of them are inert to the enzyme and the substrate, that is, they do not interfere sterically adapt to each other, while the other compounds signifcantly inhibit activity. Only two compounds containing copper ions but different ligands: hidazepam (сompound 3) and its condensation product with pyruvic acid (compound 7) showed a signifcant stimulatory effect (about 3.5 times). In the case of  peptidase 2 overall picture and the degree of infuence on the study of effector elastase and collagenase activity signifcantly different: the compounds 3 and 7 to a lesser extent stimulate their activity (1.3-1.4-fold). Analysis of data on the effect of the test compounds on fbrinolytic activity of B. thuringiensis var. israelensis IMV B-7465  peptidase 2 has shown that by using complexes 3 and 7 at a concentration of 0.001 % the enzyme activity increased to 145 % and 138 % respectively. Conclusions. It is shown that the nature of the enzyme contributes to the change in the mechanism of interaction with the substrate and effector.

Key words: peptidase 1 and 2 peptidase of Bacillus thuringiensis var. israelensis IMV B-7465, biocomplexes ions of 3d-metals (Co2+, Ni2+, Mn2+, Cu2+, Zn2+) with “gidazepam”.

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  1. Andronati S.A., Voronina T.A., Golovenko N.Ya. et al. Hydazepam. Kiev. Naukova dumka, 1992. 200 р. Russian.
  2. Bondarchyuk A.A., Azhitskii G.Yu. [Characteristics of enzyme complex from Bacillus mesentericus]. Mikrobiol. Z. 1981; 43(6):687-690. Rissian.
  3. Hugh J. Inorganic chemistry. The structure of compounds and rection ability. М.: Chemistry. 1987. 696 p. Russian.
  4. Koltukova N.V., Vaskivniuk V.T. [Selection of methods for the isolation of the proteolytic complex from Bacillus mesentericus 316m at deep cultivation]. Microbiol. Z. 1980; 42(2):245–248. Russian.
  5. Lipscomb W.N., Hartsuck J.A., Rieke G.N., Quiocho F.A., Noda L.P., Cleland W.W. Brookhaven. Symp. Biol. 1968; 21, 24.
  6. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with Folin phenol reagent. J. Biol. Chem. 1951; 193(2):265–275.
  7. Masada M. Determination of the thrombolytic activity of Natto extract. Food style. – 2004; 8(1):92–95.
  8. Маtselyukh О.V., Nidialkova N.A., Varbanets L.D. [Purifcation and physic-chemical properties of Bacillus thuringiensis ІMV В-7324 peptidase with elastolytic and fbrinolytic activity]. Ukr. Biokhim. Z. 2012; 84(6):25-36. Ukrainian.
  9. Mediantseva E.P., Vertlyb M.G., Budnikova G.K. Metal ions as enzymes effectors. Uspechi khimii. 1998; 67(3):252-260. Russian.
  10. Nidialkova N.А., Varbanets L.D., Chernyshenko V.O. Isolation and purifcation of Bacillus thuringiensis var. israelensis IМV В-7465 peptidase with specifcity toward elastin and collagen. Ukr. Biokhim. Zhurn. 2016; 88(3):18–28. https://doi.org/10.15407/ubj88.03.018
  11. Pulya A.V., Seifullina I.I., Skorokhod L.S., Efmov N.N., Ugolkova E.A., Minin V.V. [Synthesis and Characterization of Mn(II) Coordination Compounds with 2-(7-Bromo-2-oxo-5-phenyl-3H-1,4-benzdiazepin-1-yl)acetohydrazide and Its Condensation Product with Pyruvic Acid]. Russ. J. Inorg. Chem. 2015; 60(1):51-54. Russian. https://doi.org/10.1134/S0036023615010106
  12. Pulya A.V., Seifullina I.I., Skorokhod L.S., Vlasenko V.G., Levchenkov S.I., Pavlovskii V.I. [Characterization of  the Coordination Compounds of  Co(II) and Ni(II) with 2-(7-Bromo-2-oxo-5-phenyl-3H-1,4-benzodiazepin-1-yl)acetohydrazide and Condensation Product with Pyruvic Acid]. Russ. J. General. Chem. 2015; 85(1):105–111. Russian. https://doi.org/10.1134/S107036321501017X
  13. Pulya A.V., Seifullina I.I., Skorokhod L.S., Vlasenko V.G., Zubavichus Y.V. Levchenkov S.I. [Self-assembly in the  MnX2 – 2-(7-Bromo-2-oxo-5-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-1-yl)acetohydrazide – Salicylic Aldehyde systems: composition, structure, and properties of the products]. Russ. J. General. Chem. 2015; 85(5):831–837. Russian. https://doi.org/10.1134/S1070363215050205
  14. Pulya A.V., Seifullina I.I., Skorokhod L.S., Efmov N.N., Ugolkova E.A., Minin V.V. [Copper(II) Coordination Compounds with 2-(7-Bromo-2-oxo-5-phenyl-3H-1,4-ben-zdiazepin-1-yl)acetohydrazide and Products of Its Condensation with Pyruvic Acid]. Russ. J. General. Chem. 2016; 61(1):41– 5. Russian.
  15. Trombridg G.O., Moon H.D. Purifcation of human elastase. Proc. Soc. Exp. Biol. Med. 1972; 141(3):928-931. https://doi.org/10.3181/00379727-141-36903
  16. Varbanets L.D., Маtselyukh О.V., Nidialkova N.A., Аvdiyuk К.V., Gudzenko О.V., Seifullina I.I., Мasanovets G.N., Khitrich N.V. [The coordination compounds of cobalt (II, III) with dithiocarbamic acid derivatives – modifcators of hydrolytic enzyme   activity]. Biotechnologia Acta. 2013; 6(1):73-80. Ukranian. https://doi.org/10.15407/biotech6.01.073
  17. Varbanets L.D., Маtselyukh О.V., Seifullina I.I., Khitrich N.V., Nidialkova N.A., Gudzenko О.V. [Complexes of cobalt (II, III) with dithiocarbamic acid derivatives – effectors of Bacillus thuringiensis peptidase and Eupenicillium erubescens and Cryptococcus albidus α-L-rhamnosidases]. Ukr. Biokhim. Z. 2014; 86(3):49–60. Ukrainian.
  18. Varbanets L.D., Nidialkova N.A., Borzova N.V., Seifullina I.I., Martsinko E.E., Chebanenko E.A. Complexes of biscitratogermanates and biscitratostanates with metals are modifers of Bacillus thuringiensis var. іsraelensis peptidases and Penicillium canescens, Cladosporium cladosporioides and Aspergillus niger α-galactosidases activities. Biotechnologia acta. 2016; 9(3):52-60. https://doi.org/10.15407/biotech9.03.052