Mikrobiol. Z. 2017; 79(2):57-66. Ukrainian.
doi: https://doi.org/10.15407/microbiolj79.02.057

Study of Cellulose Degradation Enzymatic Complex of Penicillium funiculosum Thom Strains
from Different Habitats

Yurieva О.M., Kurchenko I.M., Syrchin S.O., Pavlychenko A.K.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Aim. Study of endo-, exoglucanase and xylanase activities on the nutrient medium with wheat straw of Penicillium funiculosum strains from different habitats. Methods. The objects of the study were 20 endophytic and soil P. funiculosum strains from culture collection of microscopic fungi of Department of Physiology and Taxonomy of Micromycetes of D.K. Zabolotny Institute of Microbiology and Virology of NASU. Endo-, exoglucanase and xylanase activities were determined quantitative by method with 3.5-dinitrosalicylic acid. Results. Both trophic groups of P. funiculosum were able to synthesize cellulolytic enzyme complex and xylanase. Endo-, exoglucanase and xylanase activities varied at the strain level and were higher for endophytic strains. The maximum values of endo-, exoglucanase and xylanase activities of endophytic strains reached 0.35 ± 0.07, 0.063 ± 0.002 and 96.1 ± 2.42 U/ml, respectively. Conclusions. Cellulase and xylanase activities of endophytic and soil P. funiculosum strains were investigated by quantitative methods for the frst time. The level of the studied enzymatic activities depended on fungal strain. Endophytic strains characterized by the maximum and minimum cellulolytic and xylanase activities.

Key words: Penicillium funiculosum, endophytes, saprophytes, endo-, exoglucanase and xylanase activities.

Full text (PDF, in Ukrainian)

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