Mikrobiol. Z. 2017; 79(1):59-65.
doi: https://doi.org/10.15407/microbiolj79.01.059

The Role of Polyphosphatases in the Cell Wall and Cytoplasmic
Membrane Response to the Action of Stress

Pidgorskyi V.S., Voychuk S.I., Gromozova E.N.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The impact of an altered phosphorus metabolism on the content of the cell wall and cytoplasmic membrane components under the infuence of physical and chemical stress factors was investigated. In this study, Saccharomyces cerevisiae strains with deleted PPN1 and PPX1 exopolyphosphatases were used. Osmotic shock, peroxide and acidic shocks, and radiofrequency electromagnetic radiation were applied to induce stress response reactions. The strains with deleted PPN1 and PPX1 had an increased content of the weakly linked and alkali–sensitive components of the cell wall proteins and a two–fold decreased content of glucosamines. The cellular fatty acid composition did not change, while the content of sterols varied depending on the strain peculiarities. The effciency and direction of stress factors action showed an individual factor–specifcity. The minor components were found to be the most labile and actively responding units to the effects of stress. That demonstrates a potential for their application as biomarkers and in industrial biotechnology. Thus, PPN1 and PPX1 polyphosphatases are involved in regulation of the biosynthesis of the cell wall and membrane components; they support their structure and are responsible for the changes in response to the infuence of stress factors.

Key words: yeast, polyphosphatases, stress, cell wall, membrane.

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