Mikrobiol. Z. 2017; 79(1):22-33.
doi: https://doi.org/10.15407/microbiolj79.01.022

Development Strategy for the New Environmentally Friendly Multifunctional Bioformulations
Based on Soil Streptomycetes

Iutynska G.O., Biliavska L.O., Kozyritska V.Ye.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Microorganism biosynthetic activity attracts researchers’ attention both in terms of the rules of various biologically active compounds synthesis by bacteria and of the industrially important microbial metabolites application in various industries and agriculture.
Scientific principles of an integrated approach to selection and study of soil streptomycetes biosynthetic potential have been formulated. Based on the approach the strains Streptomyces avermitilis ІМV Ас-5015, S. netropsis ІМV Ас-5025 and S. violaceus ІМV Ас-5027 with antagonistic activity against phytopathogens and phytonematodes were obtained. Their ability to synthesize a wide range of biologically active metabolites was shown. The latter include antiparasitic antibiotics, free amino and fatty acids, lipids, phytohormones (auxins, cytokinins, gibberellins, brassinosteroids) and others. The strategy of creation of new environmentally friendly multifunctional metabolitic bioformulations with a complex mechanism of action, combining such properties as biocontrol of phytopathogens and phytonematodes and the ability to optimize the hormonal balance and enhance plants tolerance has been substantiated theoretically and confirmed experimentally. Under the action of these bioformulations reprogramming of plant genomes occur, turning towards the synthesis induction of small regulatory si/miRNA with antipathogenic and antiparasitic properties. Under the bioformulations application the microorganisms development in root zone is activated, the overall biological activity of soil increases, the level of plant damage caused by phytopathogens and parasitic nematodes reduces, the yield increases and its quality improves.
The new bioformulations showed combined biological activity caused both by direct action on various pathogens of different etiology and by phytoregulatory activity or by increasing of plant resistance to biotic and abiotic stresses.

Key words: soil streptomycetes, antagonism, phytopathogens, phytonematodes, lipids, phytohormones, steroid compounds, metabolitic bioformulations, si/miRNA.

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