Mikrobiol. Z. 2016; 78(6):84-91.
doi: https://doi.org/10.15407/microbiolj78.06.084
Fundamental Basis of Creation of Probiotic with Provitamin Activity Based on
Strains Bacillus amyloliquefaciens IMV В-7513 and IMV B-7525
Avdeeva L.V., Kharkhota M.A., Nechypurenko O.О.
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
Dysbiotic conditions prevail in both humans and animals. Intestinal dysbiosis is accompanied, in addition to the quantitative and qualitative changes of the microflora, by immunosuppression and metabolic disorders, such as avitaminosis А. Therefore, the search and study of microorganism strains, which may be used for correction of dysbiosis and allow to normalize the homeostasis of a macroorganism, are highly relevant today. In relation to that the use of carotene synthesizing strains of bacteria of Bacillus genus is very perspective. This would allow connecting in one preparation the properties of probiotics and ability to compensate the deficiency of vitamin А in a macroorganism.
The article presents the analysis of literature data along with the results of own research concerning carotene synthesizing activity of bacteria of Bacillus genus, the nature of their carotenoid pigments and their function in a macroorganism. It was determined that carotenoid pigments of B. amyloliquefaciens ІМВ В-7513 and ІМВ В-7525 strains were presented by a complex with lipids and carbohydrates, and belonged to C30-apocarotenoids, including apo-8'-phytofluene, apo-8'-ζ-carotene, apo-8'-neurosporene and apo-8'-β-carotene-3-ol. A possible way of carotene synthesis was proposed. The end product of its pathway synthesis probably is apo-8'-β-carotene-3-ol, which is able to be metabolized into vitamin A. Earlier it was shown that the investigated strains of bacteria are safe for warmblooded animals and exhibit probiotic and provitamin activity in vivo.
The obtained results provide a fundamental basis for creation of a probiotic preparation with provitamin activity based on carotene producing strains B. amyloliquefaciens IMV B-7513 and B-7525.
Key words: bacteria of the genus Bacillus, carotenoid pigments, probiotic features, provitamin activity.
Full text (PDF, in English)
- Duc LH, Fraser PD, Tam NK. Carotenoids present in halotolerant Bacillus sporeformers. FEMS Microbiol Lett. 2006; 255: 215–224. https://doi.org/10.1111/j.1574-6968.2005.00091.x
- Khaneja R, Perez-Fons L, Fakhry S. Carotenoids found in Bacillus. Journal of Applied Microbiology. 2010; 108: 1889–1902.
- Perez-Fons L, Steiger S, Khaneja R. Identification and the developmental formation of carotenoid pigments in the yellow/orange Bacillus spore-formers. Biochimica et Biophysica Acta. 2011; 1811: 177–185. https://doi.org/10.1016/j.bbalip.2010.12.009
- Manzo N, D'Apuzzo E, Coutinho PM. Carbohydrate-active enzymes from pigmented Bacilli: a genomic approach to assess carbohydrate utilization and degradation. BMC Microbiolog. 2011; 11: 1–10. https://doi.org/10.1186/1471-2180-11-198
- Shete V, Quadro L. Mammalian metabolism of β-carotene: gaps in knowledge. Nutrients. 2013; 5: 4849–4868. https://doi.org/10.3390/nu5124849
- Carotenoids as colorants and vitamin A precursors. Technological and nutritional applications. Ed. by J.C. Baurnfeind. New York: Academic Press, 1981.
- Golembiovska SL, Matselyukh BP. The production of carotene and lycopene by mutants of Streptomyces globisporus 1912. Microbiol Z. 2008; 70(4): 45–50. Ukrainian.
- Weber RW, Anke H, Davoli P. Simple method for the extraction and reverse-phase highperformance liquid chromatographic analysis of carotenoid pigments from red yeasts (Basidiomycota, Fungi). J. Chromatography. 2007; 1145: 118–122. https://doi.org/10.1016/j.chroma.2007.01.052
- López D, Kolter R. Functional microdomains in bacterial membranes. Genes and Development. 2010; 24: 1893–1902. https://doi.org/10.1101/gad.1945010
- Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001; 25: 402–408. https://doi.org/10.1006/meth.2001.1262
- Perez-Fons L, Steiger S, Khaneja R. Identification and the developmental formation of carotenoid pigments in the yellow/orange Bacillus spore-formers. Biochimica et Biophysica Acta. 2011; 1811: 177–185. https://doi.org/10.1016/j.bbalip.2010.12.009
- Steiger S, Perez-Fons L, Cutting SM. Annotation and functional assignment of the genes for the C30 carotenoid pathways from the genomes of two bacteria: Bacillus indicus and Bacillus firmus. Microbiology. 2015; 161: 194–202. https://doi.org/10.1099/mic.0.083519-0
- Avdeeva LV, Nechypurenko OO, Kharhota MA. Probiotic abilities of carotene synthesizing strains Bacillus sp. 1.1 and B. amyloliquefaciens UCM B-5113. Microbiol Z. 2015; 77(2): 22–27. https://doi.org/10.15407/microbiolj77.02.022
- Nechypurenko OO, Kharhota MA, Avdeeva LV. The safety of carotene producing strains Bacillus sp. 1.1 and B. amyloliquefaciens UCM B-5113 for homoiothermal animals. Bulletin of Taras Shevchenko National University. Biology. 2014; 68: 21–24.
- Nechypurenko OO, Kharhota MA, Avdeeva LV. Effectivity of carotene-synthesizing strains B. subtilis IMV B-7513 and B. amyloliquefaciens UCM B-5113 for correction of experimental dysbacteriosis in mice. Bulletin of Taras Shevchenko National University. The problem of physiological function regulation. 2015; 18(1): 48–51.
- Nechypurenko OO, Kharhota MA, Avdeeva LV. Effectivity of carotene producing strains Bacillus sp. 1.1 and B. amyloliquefaciens UCM B-5113 in the chickens diet. Microbiol Z. 2015; 77(3): 2–9. https://doi.org/10.15407/microbiolj77.03.002