Mikrobiol. Z. 2016; 78(5):75-82. Ukrainian.
doi: https://doi.org/10.15407/microbiolj78.05.075

Cellulase and Xylanase Activities of Endophytic and Soil Penicillium funiculosum Strains

Yurieva О.M., Kurchenko I.M., Syrchin S.O., Kharkevych O.S., Pavlychenko A.K., Nakonechna L.T.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Aim. To carry out a comparative study of cellulase and xylanase activities of 16 endophytic and soil Penicillium funiculosum strains. Methods. The objects of the study were 16 endophytic and soil P. funiculosum strains from culture collection of microscopic fungi of Department of Physiology and Taxonomy of Micromycetes of Zabolotny Institute of Microbiology and Virology of NASU. Cellulase and xylanase activities were determined by enzyme activity zone on solid agar media with soluble cellulose (Na-CMC) and hemicellulose (xylan) and by enzymatic index (EІ). Results. All investigated P. funiculosum strains, regardless of the isolation place, can hydrolyze Na-CMC and beech xylan. Enzymatic index was studied to characterize cellulolytic and xylanolytic activities of P. funiculosum strains from different econiches for the frst time. It was established that cellulase activity of endophytic P. funiculosum strains was medium and soil ones – from low to high. Endophytic strains showed low and medium xylanase activity, while the soil strains had only high activity. Conclusions. It was established that endophytic and soil P. funiculosum strains were able to hydrolyze Na-CMC and beech xylan, while endophytes characterized by medium cellulase activity, and soil strains had from low to high level of this activity. Xylanase activity of endophytes was low, but soil isolates – only high one. Obtained experimental data might be the basis for explaining of endophytic life style of P. funiculosum in the plant, as well as in screening of producers of hydrolytic enzyme complexes for biotechnological purposes.

Key words: Penicillium funiculosum, endophytes, saprophytes, cellulase and xylanase activities, enzymatic index.

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  1. Bilay V.I., Bilay T.I., Musich E.G. Transformatsiya tsellyulozy gribami. Kyiv: Nauk. dumka, 1982.
  2. Zhdanova N.N., Zakharchenko V.A., Vasilevskaya A.I. i dr. Mikobiota Ukrainskogo Polesya: posledstviya Chernobylskoy katastrofy. Kyiv: Nauk. dumka, 2013.
  3. Koval E.Z., Mytkivska T.I. Mikolohichne obstezhennya muzeynykh pam'yatok. Kyiv: NNDRTsU, 2014.
  4. Kurakov A.V., Gevorkyan S.A., Goginyan V.B., Ozerskaya S.M. Raznoobrazie i osobennosti sostava mikroskopicheskikh gribov na sinteticheskikh polimernykh materialakh. Prikladnaya biokhimiya i mikrobiologiya. 2008; 44(2):232–235.
  5. Kurchenko I.M. Bioriznomanitnist ta ekoloho-fiziolohichni osoblyvosti endofitnykh mikromitsetiv roslyn sfahnovykh bolit Polissya Ukrainy. Kyiv, 2014.
  6. Kurchenko I.M., Zhdanova N.M., Sokolova O.V. Vyvchennya nayavnosti deyakykh hidrolitychnykh ta okysno-vidnovnykh fermentiv u shtamiv Fusarium oxysporum (Schlecht.) Snyd. et Hans., izolovanykh z riznykh mistseperebuvan. Mikrobiol. Z. 2001; 63(5):34–44.
  7. Marfenina O.E. Antropogennaya ekologiya pochvennykh gribov. Moscow: Meditsina dlya vsekh, 2005.
  8. Metody eksperimentalnoy mikologii: Spravochnik. Pod red. V.I. Bilay. Kyiv: Nauk. dumka, 1982.
  9. Mirchink T.G. Pochvennaya mikologiya. Moscow: Izd-vo Mosk. gos. un-ta, 1988.
  10. Adejuwon A.O., Oni A.O., Ajayi A.A. et al. Cellulase activity in tomato fruits infected with Penicillium funiculosum Thom. African Journal of Plant Science. 2009; 3(5):113–116.
  11. de Castro A.M., Ferreira L.S.G., Pereira N. Cellulases from Penicillium funiculosum: production, properties and application to cellulose hydrolysis. Journal of Industrial Microbiology and Biotechnology. 2010; 37(2):151–158. https://doi.org/10.1007/s10295-009-0656-2
  12. Colonia B.S.O., Chagas A.F.J. Screening and detection of extracellular cellulases (endo- and exo-glucanases) secreted by filamentous fungi isolated from soil using rapid tests with chromogenic dyes. African Journal of Biotechnology. 2014; 13(52):4694–4701. https://doi.org/10.5897/AJB2014.14221
  13. Domsch K.H., Gams W., Anderson T.-H. Compendium of soil fungi. Eching: IHW-Verlag, 2007.
  14. Florencio C., Couri S., Farinas C.S. Correlation between agar plate screening and solid-state fermentation for the production by Trichoderma strains. Enzyme Research, 2012. https://doi.org/10.1155/2012/793708
  15. Gusakov A.V., Sinitsyn A.P. Cellulases from Penicillium species for producing fuels from biomass. Biofuels. 2012; 3(4):463–477. https://doi.org/10.4155/bfs.12.41
  16. Hankin L., Anagnostakis S.L. The use of solid media for detection of enzyme production by fungi. Mycologia. 1975; 67(3):597–607. https://doi.org/10.1080/00275514.1975.12019782
  17. Jorgensen H., Olsson L. Production of cellulases by Penicillium brasilianum IBT20888–effect of substrate on hydrolytic performance. Enzyme Microbial Technology. 2006; 38(3-4):381–390. https://doi.org/10.1016/j.enzmictec.2005.06.018
  18. Khan A.L., Lee I.J. Endophytic Penicillium funiculosum LHL06 secretes gibberellin that reprograms Glycine max L. growth during cooper stress. BMC Plant Biology. 2013; 13:86. https://doi.org/10.1186/1471-2229-13-86
  19. Molitoris H.P., Schaumann K. Physiology of marine fungi. A screening program for marine fungi. In: The biology of marine fungi. Ed. S.T. Moss. Cambridge: Cambridge University Press, 1986. P. 35–47.
  20. Partida-Martínez L.P., Heil M. The microbe-free plant: fact or artifact? Frontiers in Plant Science. 2011; 100(2):1–16. https://doi.org/10.3389/fpls.2011.00100
  21. Ribeiro L.F.C., Ribeiro L.F., Jorge J.A. et al. Screening of filamentous fungi for xylanases and cellulases not inhibited by xylose and glucose. British Biotechnology Journal. 2014; 4(1):30–39. https://doi.org/10.9734/BBJ/2014/6066
  22. Robl D., da Silva Delabona P., Montanari Mergel C. et al. The capability of endophytic fungi for production of hemicellulases and related enzymes. BMC Biotechnology. 2013; 13:94. https://doi.org/10.1186/1472-6750-13-94
  23. Rodriguez R.J., White J.F., Jr, Arnold A.E. et al. Fungal endophytes: Diversity and functional roles. New Phytologist. 2009; 182(2):314–330. https://doi.org/10.1111/j.1469-8137.2009.02773.x
  24. Teather R.M., Wood P.J. Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Applied and Environmental Microbiology. 1982; 43(4):777–780.
  25. Wykyan J.P.N., Leagale P.B. Saccharification of wastepaper mixtures with cellulose from Penicillium funiculosum. Biotechnology Letters. 2001; 23(22):1849–1852. https://doi.org/10.1023/A:1012706708176