Mikrobiol. Z. 2016; 78(5):65-74. Ukrainian.
doi: https://doi.org/10.15407/microbiolj78.05.065
Influence of Pseudomonas syringae pv. atrofaciens Lipopolysaccharides on Physiological
and Biochemical Processes in Allium cepa Cells
Butsenko L.M.
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
Aim. To investigate the effect of Pseudomonas syringae pv. atrofaciens strains lipopolysaccharides (LPS) on some physiological and biochemical processes in the Allium cepa cells. Methods. LPS was extracted with 0.85 % NaCl solution. To study the phytotoxicity, onion seeds were germinated in the LPS solution and determined their germination and root length. Mutagenic activity of LPS was determined in A. cepa test. Enzyme activity and malondialdehyde content determined using classical methods. Results. It is established, that P. syringae pv. atrofaciens LPS inhibit the processes of plant cell division and induce destructive changes of chromosomes in the cells of Allium cepa root apical meristem. After treatment, the A. cepa seedlings LPS of virulent strain P. syringae pv. atrofacіens 9400 at concentrations ranging from 1.0 to 10.0 mg/ml peroxidase activity increases. LPS of avirulent strain P. syringae pv. atrofacіens 9417 signifcantly increases the peroxidase activity at concentrations 10.0; 5.0 and 2.5 mg/ml and it does not affect at a concentration 1.0 mg/ml. The onion seedlings did not observe statistically signifcant changes catalase activity when exposed to LPS solutions. The content of malonaldehyde in A. cepa seedlings increased after LPS P. syringae treatment in concentrations of 5.0 and 2.5 mg/ml. The content of this product of lipid peroxidation in these conditions exceeds the benchmark of 1.8 – 3.7 times. Conclusions. It is established that the action of LPS P. syringae pv. atrofaciens increased peroxidase activity and increases the content of malondialdehyde in the plant cells. Phytotoxic and genotoxic activity of P. syringae pv. atrofaciens LPS may be due to the appearance of reactive oxygen species under the influence of studied biologically active substances.
Key words: P. syringae pv. atrofaciens, lipopolysaccharide, genotoxic activity phytotoxic activity, reactive oxygen species.
Full text (PDF, in Ukrainian)
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