Mikrobiol. Z. 2016; 78(5):21-29. Russian.
doi: https://doi.org/10.15407/microbiolj78.05.021

Screening of Mannane-Degrading Enzymes Producers

Borzova N.V., Varbanets L.D., Kurchenko I.M., Nakonechna L.T.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The aim of research was to investigate the prevalence of complex mannan-degrading enzymes among museum and freshly isolated cultures of micromycetes, actinobacteria and bacteria. It has been shown that the producers of β-mannanases mostly extracted from sources that are rich in plant residues. We detected strains of Penicillium aculeatum, P. tardum and P. rugulosum which produce a complex of β-mannanases and α-galactosidase activity. Also, strains of thermophilic micromycetes species Corynascus sepedonium, Scytalidium thermophilum and Rhizomucor tauricus with high mannanase activity in culture liquid were detected (10 – 130 U/ml). Two strains of P. aculeatum and P. tardum showed β-mannanase, β-mannosidase and α-galactosidase activity. Actinobacteria was shown high potential, 70 % of all tested strains showed mannanase activity; their activity ranged from 2 to 55 U/ml. Among the most active bacterial cultures were representatives of soil microflora: Bacillus circulans, B. subtilis, B. mesentericus, where as among the strains isolated from sea water the active mannanases producers were not found.

Key words: β-mannanase, α-galactosidase, β-mannosidase, screening.

Full text (PDF, in Russian)

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