Mikrobiol. Z. 2016; 78(4):2-10.
doi: https://doi.org/10.15407/microbiolj78.04.002

Optimization of the Cultivation Conditions and the Basic Molecular Tools
for Roseoflavin Producer Streptomyces davawensis

Tsyrulnyk A.O.1, Kordiaka R.2, Dmytruk K.V.1, Fedorovych D.V.1, Sybirny A.A.1,2

1Institute of Cell Biology, NAS of Ukraine
14/16 Drahomanov Str., Lviv, 79005, Ukraine

2University of Rzeszow
4 Zelwerowicza St., Rzeszow, 35-601, Poland

Roseoflavin (RoF), and its metabolic precursor 8-dimethylaminoriboflavin (AF), produced by Gram positive bacteria Streptomyces davawensis and Streptomyces cinnabarinus, reveal a strong antibiotic effect against Staphyloccus aureus, Bacillus subtilis, Bacillus cereus, Micrococcus luteus and other Gram positive bacteria and could be promising for developing the new effective antibacterial drugs. In this work, we present the optimization of basic molecular tools for S. davawensis including total DNA preparation, PCR amplification of target gene, cell transformation. In addition, the compositions of cultivation media were studied and the optimal cultivation conditions for increased of RoF production have been developed.

Key words: Streptomyces davawensis transformation, roseoflavin production, nutrient media compositions, total DNA isolation, rosA gene amplification.

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