Mikrobiol. Z. 2016; 78(3):52-60. Ukrainian.
doi: https://doi.org/10.15407/microbiolj78.03.052

Physiology of Growth Pseudomonas syringae pv. atrofaciens for the Effects of Pesticides

Buletsa N.M., Butsenko L.M., Pasichnyk L.A., Patyka V.P.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The aim of this work was to study the growth of the basal bacteriosis pathogen of wheat and determination the physiological changes of Pseudomonas syringae pv. atrofaciens cells, during application of pesticides in the culture medium. Methods. The work carried out using classic microbiological methods, electron microscopy, electrophoresis, SDS PAGE. Results. It is shown that pesticides Twix and Alpha Super to reduce survival of P. syringae pv. atrofaciens UKM B-1011 in manufacturer’s recommended doses. Pyrethroid insecticides Alpha Super, Twix and Granstar Gold 75 herbicide causes morphological dissociation of colonies of P. syringae pv. atrofaciens UKM B-1011, resulting in the appearance of R-forms. All R-dissociant in physiological and biochemical characteristics were not significantly different and had minor differences from the source S-form of P. syringae pv. atrofaciens UKM B-1011. Conclusions. Therefore, synthetic pesticides Alpha Super, Twix, Granstar Gold 75 promote dissociation of P. syringae pv. atrofaciens UKM B-1011, thereby increasing the heterogeneity of the bacterial populations and expanding the boundaries of endurance of specie.

Key words: Pseudomonas syringae pv. atrofaciens, pesticides, R-forms, morphological dissociation.

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