Mikrobiol. Z. 2016; 78(3):36-44. Ukrainian.
doi: https://doi.org/10.15407/microbiolj78.03.036

Polyphasic Taxonomic Analysis of Carotene-Synthesizing Strain Bacillus sp. 1.1,
Perspective for the Creation of Probiotic

Nechypurenko O.O., Kharhota M.A., Zelena L.B., Ostapchuk A.M., Avdeeva L.V.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

It was done the polyphasic taxonomic analysis of carotene synthesizing strain Bacillus sp. 1.1, perspective for the creation of probiotic. The investigated strain was referred to the species Bacillus subtilis based on of cultural-morphological, physiological and biochemical features. Despite this the fatty acid profile of the cell walls of this strain is predominantly presented by branched derivatives of iso- and antiiso C15:0 and C17:0 compounds (over 70 %), which is typical for the species Bacillus amyloliquefaciens. Based on the nucleotide sequence of 16S rRNA gene, and the profile of polymorphic nucleotides strain was referred to the species Bacillus amyloliquefaciens.

Key words: strain Bacillus sp., polyphasic taxonomic analysis.

Full text (PDF, in Ukrainian)

  1. Avdyeyeva L.V., Nechypurenko O.O., Kharkhota M.A. Probiotychni vlastyvosti karotynsyntezuvalnykh shtamiv Bacillus sp. 1.1 ta B. amyloliquefaciens UKM V-5113. Mikrobiol. Z. 2015; 77(2):22-27.
  2. Nechypurenko O.O., Kharkhota M., Avdyeyeva L., Zelena L. Pryroda ta fizyko-khimichni vlastyvosti karotynoidnykh pih­mentiv shtamiv Bacillus sp. 1.1 ta B. amyloliquefaciens UKM V-5113. XI Ukrainskyi biokhimichnyi konhres, 6-10 zhovtnya 2014 roku, Kyiv, 2014. P. 34.
  3. Pokhilenko V.D., Perelygin V.V. Probiotiki na osnove sporoobrazuyushchikh bakteriy i ikh bezopas­nost. Khimicheskaya i biologicheskaya bezopas­nost. 2007; 2:32-33.
  4. Smirnov V.V., Reznik S.R., Sorokulova I.B. Metodicheskie rekomendatsii po vydeleniyu i identifikatsii bakteriy gruppy Bacillus subtilis-mesentericus iz organizma cheloveka i zhivotnykh. Kyiv: Naukova dumka, 1980.
  5. Ushakova N.A., Nekrasov R.V., Pravdin V.G. Novoe pokolenie probioticheskikh preparatov kormovogo naznacheniya. Fundamentalnye issledovaniya. 2012; 1:184-193.
  6. Amin M., Rakhisi Z., Ahmady A.Z. Isolation and identification of Bacillus species from soil and evaluation of their antibacterial properties. J. Clin. Microb. Infec. 2015; 2:1-4.
  7. Berkeley R., Heyndrickx M., Logan N. Applications and systematics of Bacillus and relatives. Wiley-Blackwell, 2002. https://doi.org/10.1002/9780470696743
  8. De Vos P., Garrity G.M. et al. The Firmicutes. Bergey's manual of systematic bacteriology. New York: Springer, 2009.
  9. Ehrhardt C.J., Chu V., Brown T.C., Simmons T.L., Swan B.K., Bannan J., Robertson J.M. Use of fatty acid methyl ester profiles for discrimination of Bacillus cereus T-Strain spores grown on different media. Appl. Envir. Microbiol. 2010; 76(2):1902-1912. https://doi.org/10.1128/AEM.02443-09
  10. Erem F., Certel M., Karakas B. Identification of Bacillus species isolated from ropey breads both with classical methods and api identification kits. Akdeniz Universitesi Ziraat Fakultesi Dergisi. 2009; 22:201-210.
  11. Flores O., Belanche L.A., Blanch A.R. New multiplatform computer program for numerical identification of microorganisms. J. Clin. Microbiol. 2009; 47:4133-4135. https://doi.org/10.1128/JCM.01250-09
  12. Hwang K.Y., Cho J.H., Lee J. Y., Kang K.D. The benefits of using bacillus as a probiotic. Journal of animal and veterinary advances. 2012; 18:3457-4362.
  13. Lane D.G. Nucleic acids techniques in bacterial systematic. Eds. by E. Stackebrandt, M. Goodfellow. Chichester, United Kingdom: John Wiley, 1991. P. 115-175.
  14. Perez-Fons L., Steiger S., Khaneja R. Identification and the developmental formation of carotenoid pigments in the yellow/orange Bacillus spore-formers. Biochimica et Biophysica Acta. 2011; 1811:177-185. https://doi.org/10.1016/j.bbalip.2010.12.009
  15. Reva O.N., Sorokulova I.B., Smirnov V.V. Simplified technique for identification of the aerobic spore-forming bacteria by phenotype. International Journal of Systematic and Evolutionary Microbiology. 2001; 51:1361-1371. https://doi.org/10.1099/00207713-51-4-1361
  16. Safronova L.A., Zelena L.B., Klochko V.V., Reva O.N. Does the applicability of Bacillus strains in probiotics rely upon their taxonomy. Can. J. Microbiol. 2012; 58(2):212-219. https://doi.org/10.1139/w11-113
  17. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution. 2011; 28:2731-2739. https://doi.org/10.1093/molbev/msr121