Mikrobiol. Z. 2016; 78(2):74-79. Ukrainian.
doi: https://doi.org/10.15407/microbiolj78.02.074

Resistance to Dehydratation of Extremophilic Bacteria from Antarctic Region and Hypersaline Reservoirs

Romanovskaya V.A., Rokitko P.V., Gladka G.V., Tashyrev A.B.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Purpose. To determine resistance to dehydratation of bacteria resistant to UV irradiation. Methods. Objects of research - extremophilic bacteria (from Antarctic islands and hypersaline reservoirs of Israel and Crimea). Dehydratation of bacteria was carried out in sealed desiccator; the exposure without access of moisture was 5 weeks, temperature - 22 - 25 °C, humidity - 1 - 4 %. UV irradiation of bacteria was carried out by lamp BUF 15 (A = 254 nm) in the range of 40 - 2400 J/m2. After cultivation irradiated and not irradiated strains (before and after UV irradiation and before and after drying) we determined the number of cells. Results. The extremely resistant to dehydratation (Firmicutes) and extremely sensitive to dehydratation (Proteobacteria, Bacteroidetes) strains was found among the studied bacteria. Most strains of bacteria that were resistant to dehydratation were also resistant to UV irradiation. Survival after dehydratation in these bacteria was 6.32 - 55.26 % and UV LD99.99 240-600 J/m2. Instead, representatives of Proteobacteria were extremely sensitive to these extreme factors: the cell survival was 0 - 1.6 % after dehydratation and UV LD99.99 80 - 180 J/m2. Conclusions. The results support the hypothesis that between bacteria resistance to electromagnetic radiation (UV) and their resistance to dehydratation there is a correlation.

Key words: extremophilic bacteria, resistance, dehydratation, UV irradiation.

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