Mikrobiol. Z. 2016; 78(2):21-32. Ukrainian.
doi: https://doi.org/10.15407/microbiolj78.02.021

The Effect of Metal Ions and Chemical Reagents on the Activity of Achromobacter sp. 7a α-Amylase

Avdiyuk K.V., Varbanets L.D.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The effect of cations and anions on the activity of Achromobacter sp. 7a α-amylase was studied. It is shown that tested enzyme is stable to most of anions, however sensitive to a number of cations. The most significant inhibitory effects on the activity of Achromobacter sp. 7a α-amylase exerted Hg2+, Al3+, Fe3+, Cu2+ and Ag+ ions. Decline of activity Achromobacter sp. 7a α-amylase in the presence of EDTA and EGTA indicated on the presence within its structure of metal ions. An important role in the functioning of this enzyme play a carboxyl and sulfhydryl groups as evidenced by its inhibition of 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide methiodide and p-chloromercuribenzoate respectively. α-Amylase Achromobacter sp. 7a does not contain histidine imidazole group in the active center, unlike most studied glycosidases. The tested enzyme showed high stability in the presence of Tween-20, urea, peroxide of hydrogen, making it competitive with previously described α-amylases.

Key words: α-amylase, Achromobacter sp., metal ions, chemical reagents, photooxidation, detergents.

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