Mikrobiol. Z. 2016; 78(2):13-20. Ukrainian.
doi: https://doi.org/10.15407/microbiolj78.02.013
Phylogenetic Analysis of Gene 16S rRNA Nucleotide Sequence of Probiotic Strain Lactobacillus sp. 55
Ogirchuk K.S., Kovalenko N.K.
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
The identification of probiotic strain Lactobacillus sp. 55 at species level on the basis of analysis of the 16S rRNA gene nucleotide sequences has been done. It has been established that investigated strain belongs to the species Lactobacillus gasseri. The nucleotide sequence of strain Lactobacillus gasseri 55 16S rRNA gene’s fragment has been deposited in international database GenBank (NCBI) under number 314159 KT.
Key words: 16S rRNA gene sequencing, Lactobacillus delbrueckii-acidophilus group.
Full text (PDF, in Ukrainian)
- Dzhobulaeva A.K., Sadanov A.K., Aytkeldieva S.A., Baykara B.T., Dzhakibaeva G.T., Kebekbaeva K.M. Molekulyarno-geneticheskaya identifikatsiya dvukh shtammov molochnokislykh bakteriy na osnove analiza nukleotidnykh posle dovatelnostey 16S rRNA gena. Mezhdunarodnyi zhurnal prikladnykh i fundamentalnykh issledovaniy. 2014; 8:63-67.
- Ohirchuk K.S., Kovalenko N.K., Poltavska O.A. Identyfikatsiya ta biolohichni vlastyvosti molochnokyslykh bakteriy, izolovanykh vid zhinok starshykh vikovykh hrup. Mikrobiol. Z. 2013; 75(5):3-9.
- Ohirchuk K.S., Poltavska O.A., Kovalenko N.K. Antahonistychni vlastyvosti molochnokyslykh bakteriy, vydilenykh vid zhinok praktychno zdorovykh ta khvorykh na osteoporoz. Mikrobiol. Z. 2013; 75(1):21-27.
- Berger B., Pridmore R.D., Barretto C., Delmas-Julien F., Schreiber K., Arigoni F., Brussow H. Similarity and differences in the Lactobacillus acidophilus group identified by polyphasic analysis and comparative genomics. J. Bacteriol. 2007; 189:1311-1321. https://doi.org/10.1128/JB.01393-06
- Chandok H., Shah P., Akare U.R., Hindala M., Bhadoriya S.S., Ravi G.V., Sharma V., Bandaru S., Rathore P., Nayarisseri A. Screening, Isolation and Identification of Probiotic Producing Lactobacillus acidophilus Strains EMBS081 & EMBS082 by 16S rRNA Gene Sequencing. Interdiscip. Sci. Comput. Life Sci. 2014; 6:1-6.
- Cousin S., Motreff L., Gulat-Okalla M-L., Gouyette C., Sproer C., Schumann P., Begaud E., Bouchier C., Clermont D., Bizet C. Lactobacillus pasteurii sp. nov. and Lactobacillus hominis sp. nov. International Journal of Systematic and Evolutionary Microbiology. 2013; 63:53-59. https://doi.org/10.1099/ijs.0.036665-0
- Devereux R., Wilkinson S.S. Amplification of ribosomal RNA sequences. Molecular Microbial Ecology Manual, Second Edition. 2004; 3(1):509-522.
- Donelli G., Vuotto C., Mastromarino P. Phenotyping and genotyping are both essential to identify and classify a probiotic microorganism. Microbial Ecology in Health & Disease. 2013; 24:20105. https://doi.org/10.3402/mehd.v24i0.20105
- Fujisawa T., Benno Y., Yaeshima T., Mitsuoka T. Taxonomic Study of the Lactobacillus acidophilus group, with recognition of Lactobacillus gallinarum sp. nov. and Lactobacillus johnsonii sp. nov. and synonymy of Lactobacillus acidophilus group A3 (Johnson et al. 1980) with the type strain of Lactobacillus amylovorus (Nakamura 1981). International journal of systematic bacteriology. 1992; 42(3):487-491. https://doi.org/10.1099/00207713-42-3-487
- Huang C.-H., Chang M.-T., Huang M.-C., Wanga L.-T., Huanga L., Lee F.-L. Discrimination of the Lactobacillus acidophilus group using sequencing, speciesspecific PCR and SNaPshotmini-sequencing technology based on the recA gene. J. Sci. Food Agric. 2012. https://doi.org/10.1002/jsfa.5692
- Johnson J.L., Phelps C.F, Cummins C.S., London J., Gasser, F Taxonomy of the Lactobacillus acidophilus group. Int. J. Syst. Bacteriol. 1980; 30:53-68. https://doi.org/10.1099/00207713-30-1-53
- Kullen M.J., Sanozky-Dawes R.B., Crowell D.C., Klaenhammer T.R. Use of the DNA sequence of variable regions of the 16S rRNA gene for rapid and accurate identification of bacteria in the Lactobacillus acidophilus complex. Journal of Applied Microbiology. 2000; 89:511-516. https://doi.org/10.1046/j.1365-2672.2000.01146.x
- Lactic Acid Bacteria: Biodiversity and Taxonomy, First Edition. Edited by W.H. Holzapfel, B.J.B. Wood. John Wiley & Sons, Ltd., 2014.
- Pessione E. Interactive Probiotics. CRC Press, 2014. https://doi.org/10.1201/b16439
- Sarmiento-Rubiano L-.A., Berger B., Moine D., Zuniga M., Perez-Martmez G., Yebra M.J. Characterization of a novel Lactobacillus species closely related to Lactobacillus johnsonii using a combination of molecular and comparative genomics methods. BMC Genomics. 2010; 11:504.
https://doi.org/10.1186/1471-2164-11-504 - Wang L.-T., Kuo H.-P., Wu Y.-C., Tai C.-J., Lee F.-L. Lactobacillus taiwanensis sp. nov., isolated from silage. Int. J. Syst. Evol. Microbiol. 2009; 59:2064-2068. https://doi.org/10.1099/ijs.0.006783-0
- Multalin - http://multalin.toulouse.inra.fr/multalin
- Sequence Manipulation Suite - http://www.bioinformatics.org/sms2
- BLAST, GenBank - http://www.ncbi.nlm.nih.gov/BLAST