Mikrobiol. Z. 2016; 78(2):2-12. Ukrainian.
doi: https://doi.org/10.15407/microbiolj78.02.002

Biological Properties of Nocardia vaccinii IMV B-7405 Surfactants Synthesized on Fried Sunflower Oil

Pirog T.P.1,2, Nikituk L.V.1, Tymoshuk K.V.1, Shevchuk T.A.2, Iutynska G.O.2

1National University of Food Technologies
68 Volodymyrska Str., Kyiv, 01601, Ukraine

2Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Aim. To study of antimicrobial and antiadhesive properties of the surface-active substances (surfactants) Nocardia vaccinii IMV B-7405 synthesized on different oil-containing substrates (refined and waste after frying meat and potatoes sunflower oil). Methods. Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2 : 1). Antimicrobial against bacteria and yeast properties of the surfactant was determined by index of the minimum inhibitory concentration (MIC). The number (%) of attached cells (adhesion) was determined as a ratio of the optical density of the suspension obtained from the materials treated with surfactants to the optical density of the control samples (100 %). Results. It was established that surfactants synthesized N. vaccinii IMV B-7405 on fried oil, at a concentration of 0.02 - 0.04 mg/ml decreased adhesion of bacteria (Escherichia coli IEM-1, Bacillus subtilis BT-2) on plastic, dutch tile, glass and linoleum by 25-90 %, and yeast Candida albicans Д-6 - by 15 - 65 %. The minimum inhibitory concentration with respect to studied test cultures surfactants synthesized on waste after frying potato oil was 8 - 67 mg/ml, and against phytopathogenic bacteria Pectobacterium carotovorum UCM B-1095, Pseudomonas syringae pv. atrofaciens UCM B-1015 and Xanthomonas campestris pv. campestris UCM B-1049 - 14 - 52 mg/ml. Conclusions. Replacing traditional substrates on fried oil can not only reduce cost of N. vaccinii IMV B-7405 surfactant biosynthesis, but also to obtain the final product with high antiadhesive and antimicrobial properties. The dependence of antiadhesive and antimicrobial potential of surfactants on the nature of the carbon source indicate the need for studies effect of cultivation conditions of producer on biological properties of synthesized surfactants.

Key words: Nocardia vaccinii IMV B-7405, surfactants, conditions of cultivation, antimicrobial and antiadhesive properties, fried oil.

Full text (PDF, in Ukrainian)

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