Mikrobiol. Z. 2016; 78(2):111-118. Ukrainian.
doi: https://doi.org/10.15407/microbiolj78.02.111

Phylogenetic Analysis of Ukrainian Isolate of Beet Necrotic Yellow Vein Virus

Hrynchuk K.V.1, Antipov I.A.1, Parii M.F.1, Kyrychenko A.M.2

1National University of Life and Environmental Sciences of Ukraine
15 Heroyiv Oborony Str., Kyiv, 03041, Ukraine

2Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The analysis of Ukrainian isolate of beet necrotic yellow vein virus has been performed. The partial nucleotide sequence of cDNA corresponding to RNA-2 of BNYVV isolates were analyzed and Ukrainian isolate AG9 of BNYVV was assigned to type A strains based on DNA sequences. The nucleotide sequence of gene encoding a coat protein of Ukrainian isolate of BNYVV was compared with appropriate nucleotide sequences existing in the GeneBank and the phylogenetic analysis of investigated virus was done. It was shown that Ukrainian isolate AG9 of BNYVV has 100 % homology to isolate originating from Sweden.

Key words: beet necrotic yellow vein virus, Ukrainian isolate, polymerase chain reaction (PCR), coat protein (CP), RNA-2, phylogenetic analysis.

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