Mikrobiol. Z. 2016; 78(1):33-43.
doi: https://doi.org/10.15407/microbiolj78.01.033

The Regularities of Iron Compounds Transformation by Citrobacter freundii Ml-31.1/1

Govorukha V.M., Tashyrev O.B.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The purpose of the work was to study the regularities of Fe(III) compounds reduction under the conditions of limited aeration by the strain Citrobacter freundii Ml-31.1/1, and to assess the role of Fe(III)-reducing bacteria (FRB) in iron compounds transformation in ecosystems. The following methods were used: cultivation of microorganisms under the conditions of limited aeration, colorimetric determination of biomass and Fe(III) and Fe(II) compounds concentration, the potentiometric measuring of pH and Eh, gas chromatography. Results: Thermodynamic calculations of microbial interaction with iron compounds were experimentally verified. The regularities of Fe(III) and Fe(II) compounds transformation by the model strain Citrobacter freundii Ml-31.1/1, isolatedfrom the typical mid-latitude ecosystem - river sludge were obtained. The balance of iron compounds redistribution in cells and in the extracellular fraction was established. Conclusions: The results obtained are the basis to assume that non-specific interaction of microorganisms with iron may significantly influence transformation of iron. Thermodynamic prognosis allows to carry out targeted regulation of microbial metabolism.

Key words: thermodynamic prognosis, microbial Fe(III) reduction, precipitation of iron compounds, biogeochemical cycles of iron transformation.

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