Mikrobiol. Z. 2015; 77(4):25-31. Ukrainian.
doi: https://doi.org/10.15407/microbiolj77.04.025

Optimization of Cultivation Conditions of Penicillium tardum - the α-L-Rhamnosidase Producer

Gudsenko O.V., Varbanets L.D.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The influence of some technological cultivation parameters of Penicillium tardum to synthesize of the extracellular α-L-rhamnosidase were studied. It was shown that rhamnose (0.8 %), yeasts autolysate (0.2 %), temperature of the cultivation 25 °C, pH 5.0 are necessary for maximal α-L-rhamnosidase production. The enzyme reaches the maximal activity level in 96 hours with sulphitic number equal 0.44. At cultivation of P. tardum in the picked up conditions the α-L-rhamnosidase synthesis has raised in 4 times.

Key words: Penicillium tardum, α-L-rhamnosidase, the optimization of the cultivation conditions, carbon and nitrogen sources.

Full text (PDF, in Ukrainian)

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