Mikrobiol. Z. 2015; 77(3):36-46.
doi: https://doi.org/10.15407/microbiolj77.03.036

Virion Morphology and Structural Organization of Polyvalent
Bacteriophages ТT10-27 and КEY

Faidiuk I.V., Boyko A.A., Muchnyk F.V., Tovkach F.I.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Fine ultrastructure of polyvalent bacteriophages TT10-27 and KEY, isolated from affected with fire blight disease plant tissues, was studied using electron microscopy. Phages have isometric heads connected to short complex tail (TT10-27, Cl-morphotype) or long non-contractile tail (KEY, B-1 morphotype). Maximum diameter of TT10-27 head, measured as the distance between opposite vertices, is 71.3 nm; tail tube of 22 nm in diameter and 9.0 nm in width is framed with 12 appendages that form flabellate structure of 47.0-58.6 nm in diameter. KEY features capsid of 78.6 nm in diameter and flexible non-contractile tail of 172.5 nm long, which ends with a conical tip. Due to a number of features phage TT10-27 was assigned to a group of N4-like phages of Podoviridae family. KEY is a representative of family Siphoviridae, the least freaquent group of Erwinia amylovora phages.

Key words: bacteriophages, Erwinia amylovora, morphology and structural organization of virion.

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