Mikrobiol. Z. 2015; 77(3):28-35. Russian.
doi: https://doi.org/10.15407/microbiolj77.03.028

Bioconversion of Crude Glycerole and Molasses Mixture
in Biosurfactants of Nocardia vaccinii IMB B-7405

Pirog T.P.1,2, Kudrya N.V.1, Shevchuk T.A.2, Beregova K.A.1, Iutynska G.O.2

1National University of Food Technologies
68 Volodymyrska Str., Kyiv, 01601, Ukraine

2Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

The possibility of replacing glucose and pure glycerol in mixed substrates for surface-active substances (SAS, biosurfactants) biosynthesis of Nocardia vaccinii IMB B-7405 on molasses (sugar production waste) and crude glycerol (by-product of biodiesel production) was established.
It was established that the increasing concentration of crude glycerol to 6% in mixture with 1.0 % molasses was accompanied by increase of amount of SAS synthesized more than twice, and he increasing content of molasses to 3,0 % in mixture with 1,0 % crude glycerol - by some decrease n the level of surfactant as compared to that in a medium containing 1,0% monosubstrates.
It was shown that the increasing concentration of sodium nitrate to 2-fold in medium cultivation of N. vaccinii IMB B-7405 allowed to increase to 7,0 % content of grude glycerol in mixture with 1.0 % molasses. Under such conditions of cultivation concentration of exocellular SAS synthesized was 7,5 g/l, that to 1,3 fold higher than in basic medium with a lower content of nitrogen source.

Key words: biosurfactants, Nocardia vaccinii IMB B-7405, biosynthesis, industrial waste, mixture of substrates.

Full text (PDF, in Russian)

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