Mikrobiol. Z. 2015; 77(3):16-22. Ukrainian.
doi: https://doi.org/10.15407/microbiolj77.03.016

Ability of Microorganisms from Different Ecological Niches to Hydrolyze the Insoluble Proteins

Matseliukh O.V.1, Nidialkova N.А.1, Varbanets L.D.1, Andreeva N.O.2, Shepelevych V.V.3, Zelena P.P.3, Yumyna J.M.3

1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

2State Oceanarium, Scientific-Research Center of the Armed Forces of Ukraine
7 Epronivska Str., Sevastopol, 99024, Ukraine

3Taras Shevchenko National University of Kyiv
60 Volodymirska Str., Kyiv, 01033, Ukraine

Screening of protease producers with specificity to insoluble and hard soluble protein substrates of animal origin (collagen, fibrin, elastin and keratin) was carried out. It was studied the bacterial cultures (24 strains) isolated from water and periphyton of enclosures with dolphins, and also from exhalations, oral cavity and skin of dolphins. Some bacterial strains isolated from water and periphyton of enclosures hydrolyzed collagen (5 - 23 U/ml) and elastin (20 - 32 U/ml). Thus all tested cultures did not possess the property of extracellular keratinases synthesis. The streptomycetes (48 strains) were isolated from the soil of Black Sea coastal strip near Odessa and Saky, from parkland and the shores of freshwater lake in Saky and from the soil of Atlantic Ocean coastal strip near Albufena (Portugal). Several streptomycetes have been found to appeare the perspective producers of extracellular keratinase and collagenase. The strains isolated from the soil of the coastal strip area both sea and freshwater lake in Saky possessed the highest activity (up to 5 U/mg).

Key words: bacterial isolates, streptomycetes, collagenase, elastase, keratinase, fibrinolytic peptidase.

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