Mikrobiol. Z. 2015; 77(2):9-14. Ukrainian.
doi: https://doi.org/10.15407/microbiolj77.02.009

The Taxonomic Study of the Strain Bacillus sp. UCM B-7404 - Phytopathogenic Fungi Antagonist

Zhukova D.A.1, Klochko V.V.1, Zelena L.B.1, Reva O.M.2, Dragovoz I.V.1, Avdeeva L.V.1

1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

2University of Pretoria
Lynnwood Rd., Hillcrest, Pretoria, South Africa

The polyphas taxonomic analysis of strain Bacillus sp. UCM B-7404 active against phytopathogenic fungi and producing extracellular phytohormones, lytic enzymes and lipopeptide antifungal compounds has been carried out. The basic cell wall fatty acids presented by branched iso- and anteiso- C15:0 and C17:0 acids, contained 65 - 77 % of the average pool.
Phylogenetic assay of 16S rRNA gene nucleotide sequence, phenotypic and chemotaxonomic characteristics proved the attachment of strain Bacillus sp. UCM B-7404 to Bacillus amyloliquefaciens subsp. plantarum.

Key words: bacteria of Bacillus genus, polyphase taxonomic assay, Bacillus amyloliquefaciens species.

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