Mikrobiol. Z. 2021; 83(2):32-41.
Biosynthesis of Polyene Antibiotics and Phytohormones by Streptomyces netropsis IMV Ac-5025
under the Action of Exogenous Isopentenyladenosine
M.I. Loboda, L.O. Biliavska, G.O. Iutynska
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
Streptomyces are active producers of a wide range of metabolites with multidirectional biological activity. Streptomyces netropsis IMV Ac-5025 synthesizes a polyene antibiotic complex in which two fractions were identified: heptaene candidine and a new tetraene fraction of unknown structure. The influence of secondary metabolites on the polyene antibiotics biosynthesis by soil streptomycetes is insufficiently explored. The aim of this work was to research the effect of exogenous isopentenyladenosine on the biosynthesis of polyene antibiotics and cytokinins by S. netropsis IMV Ac-5025. Methods. The strain was cultured in submerged cultivation condition in organic (soy) and synthetic (starch-ammonia) liquid nutrient media. The studies of biomass accumulation (gravimetric method), glucose consumption (glucose oxidase method), pH changes of culture media (ionometric method), biosynthesis of polyene antibiotics, and phytohormones (quantitative and qualitative thin layer chromatography spectrodensitometric method) were conducted. The results were analyzed in Statisticav.6.0 program. Results. It was found that polyene antibiotics are synthesized after the first day of cultivation, which indicates their role in the metabolism of streptomycetes. The biggest amount of the polyene antibiotics was accumulated in the stationary phase of producer growth (on the 7th day). It was found the decrease of polyene antibiotics and cytokinins accumulation in the producer’s biomass with the increase of exogenous cytokinin concentration from 25 ng/mL to 500 ng/mL. The bioproduction of the tetraene fraction was suppressed to a greater extent – up to 92% in the synthetic and up to 23% – in organic nutrient media. However, the amount of producer biomass increased under the action of the exogenous substance that confirming the positive effect of exogenous cytokinin on cell division of S. netropsis IMV Ac-5025. Exogenous isopentenyladenosine reduced the accumulation of endogenous cytokinins in streptomycetes biomass. Conclusions. The obtained results indicate an indirect metabolic relationship between the biosynthesis of polyene antibiotics and cytokinins in soil streptomycetes and provide a basis for the regulation of the biotechnological process for bioproduct formation with the appropriate quantitative composition of its components.
Keywords: Streptomyces netropsis, polyene antibiotics, candidine, tetraene, biosynthesis of metabolites, cytokinins, isopentenyladenosine.
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- Franco-Correa M, Chavarro-Anzola V. Actinobacteria as plant growth-promoting rhizobacteria. Actinobacteria. Basics and Biotechnological Applications. 2016; 10:249–270. https://doi.org/10.5772/61291
- Procopio RE, Silva IR, Martins MK, Azevedo JL, Araujo JM. Antibiotics produced by Streptomyces. Braz J Infect Dis. 2012; 16(5):466–471. https://doi.org/10.1016/j.bjid.2012.08.014
- Biliavska LO, Pidlypska VA, Kozyrytska VY, Iutynska GO. Biosynthetical activity of soil streptomycetes – antagonists of plant-parasitic nematodes and phytopathogens. In: Proceedings of the 4th European Conf of Biology and Medical Sciences. 2015; Vienna, Austria. p. 10–17.
- Biliavska LO, Efrenkova EV, Zenkova VA, Kozyrytska VE, Iutynska GO. [Soil streptomycete Streptomyces netropsis – producer of fungicidal substances]. In: Modern mycology in Russia: The 3rd International Mycological Forum; 2015; Moscow, Russia, p. 175–177. Russian.
- Biliavska LO, Efymenko TO, Efremenkova OV, Iutynska GO. [Identification and antagonistic properties of the soil Streptomyces netropsis sp. 100]. Mikrobiol Z. 2016; 78(2):61–73. Russian. https://doi.org/10.15407/microbiolj78.02.061
- Spurgeon D. Antibiotics database launched. The BMJ. 2001; 322(7291):881. https://doi.org/10.1136/bmj.322.7291.881
- Hamilton-Miller JM. Chemistry and biology of the polyene macrolide antibiotics. Bacteriol Rev. 1973; 37(2):166–196. https://doi.org/10.1128/BR.37.2.166-196.1973
- Sousa JAJ, Olivares FL. Plant growth promotion by streptomycetes: ecophysiology, mechanisms and applications. Chem Biol Technol Agric. 2016; 3(24):1–12. https://doi.org/10.1186/s40538-016-0073-5
- Loboda MI, Voychuk SI, Biliavska LO. [Correlation dependence of the antibiotic compounds biosynthesis and other biologically active substances in soil streptomycetes]. Mikrobiol Z. 2019; 81(5):36–47. Ukrainian. https://doi.org/10.15407/microbiolj81.05.036
- Tsavkelova EA, Klimova SY, Cherdyntseva TA, et al. Microbial producers of plant growth stimulators and their practical use: A review. Appl Biochem Microbiol. 2006; 42:117–126. https://doi.org/10.1134/S0003683806020013
- Semenow SM. [Laboratory media for actinomycetes and fungi]. Directory. Moscow: Agroprom publ.; 1990. Russian.
- Iutynska GO, Biliavska LO, Kozyrytska VE. Zabolotny Institute of Microbiology and Virology, NASU. Strain S. marinolimosus – antagonist of phytopathogenic microorganisms. Patent of Ukraine 107122. 2014; Nov 25.
- Sharshunova V, Schwartz V, Mikhalets N. [Thin layer chromatography in pharmacy and clinical biochemistry]. T1. Moscow: Mir; 1980. Russian.
- Savinsky SV, Dragovoz IV, Pedchenko VK. [Determination of zeatin, indolyl-3-acetic and abscisic acids from one plant sample using high-performance liquid chromatography]. Physiol and biochem cult plants. 1991; 23(6):606–614. Russian.
- Harir M, Bendif H, Bellahcene M, Fortas Z, Pogni R. Streptomyces secondary metabolites. Basic Biology and Applications of Actinobacteria. Chapter 10. InTechPublishers; 2018. p. 99–122. https://doi.org/10.5772/intechopen.79890
- Hwang I, Sakakibara H. Cytokinin biosynthesis and perception. Physiol Plant. 2006;126(4):528–538. https://doi.org/10.1111/j.1399-3054.2006.00665.x
- Kakimoto T. Biosynthesis of cytokinins. J Plant Res. 2003;116(3):233–239. https://doi.org/10.1007/s10265-003-0095-5
- Kanehisa M, Goto S. KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Res. 2000; 28:27–30. https://doi.org/10.1093/nar/28.1.27
- Steinman ID, Safferman RS, Lechevalier H. Role of inorganic salts and mevalonic acid in the production of a tetraenic antifungal antibiotic. Antibiotic Ann. 1958; 869–873.
- Kong D, Lee MJ, Lin S, Kim ES. Biosynthesis and pathway engineering of antifungal polyene macrolides in actinomycetes. J Ind Microbiol Biotechnol. 2013; 40(6):529–543. https://doi.org/10.1007/s10295-013-1258-6
- Frebort I, Kowalska M, Hluska T, Frebortova J, Galuszka P. Evolution of cytokinin biosynthesis and degradation. J Exp Bot. 2011; 62(8):2431–2452. https://doi.org/10.1093/jxb/err004
- Calderone CT. Isoprenoid-like alkylations in polyketide biosynthesis. Nat Prod Rep. 2008; 25(5):845–853. https://doi.org/10.1039/b807243d