Mikrobiol. Z. 2020; 82(5):58-64. Ukrainian.
Virus Infected Bean Tissue Culture Cells and It’s Healing in vitro
Using Liposomal form of Glycanes
O.Hr. Kovalenko, A.M. Kyrychenko, O.Yu. Kovalenko
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
The aim of the study was to develop a recovery means for beans infected by Bean yellow mosaic virus (BYMV) as well as Bean common mosaic virus (BCMV) using callus culture and liposomal glycan preparations. Methods. Cultivation of explants and callus cultures was carried out in vitro using conventional methods of plant biotechnology. The tissue culture propagation was performed during the spring or summer seasons. The presence of viral infection was tested by reverse transcription polymerase chain reaction. The virus-specific primers that allowed amplifying the conserved regions of the capsid protein gene of BCMV or BYMV were used for virus identification. Results. The culture of bean callus infected with BCMV was obtained and adapted for antiviral agents testing. It has been shown that during long-term cultivation (10–12 weeks) in the presence of liposomal preparation containing Ganoderma adspersum glucan (10–100 mg/l), plant tissue culture become free from viruses following virus eradication. This is evidenced by the absence in the callus tissue of 391 bp sequences typical for the virus coat protein gene. Conclusions. The full suppression of virus reproduction and gradual elimination of virus occurred in callus tissue obtained from BCMV-infected beans and cultured on B-5 medium supplemented with liposomal glycanglycolipid complex (10–100 mg/l). The data obtained can be useful for the development of practical control method to cure plant virus diseases using callus culture and antiviral-active glycan-glycolipid complexes.
Keywords: callus culture, liposome, Bean yellow mosaic virus, Bean common mosaic virus.
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