Mikrobiol. Z. 2020; 82(3):45-54. Ukrainian.
Fungicides Toxicity Assessment on Bradyrhizobium japonicum Nodule Bacteria in Pure Culture
N.А. Vorobey, K.P. Kukol, S.Ya. Kots
Institute of Plant Physiology and Genetics, NAS of Ukraine
31/17 Vasylkivska Str., Kyiv, 03022, Ukraine
Aim. To estimate the degree of Bradyrhizobium japonicum nodule bacteria strains sensitivity to systemic-contact fungicides Maxim XL, Standak Top, Fever, Akanto Plus and Benorad, which will help to resolve the issue of compatibility of plant protection chemicals with inoculation of seeds with bacterial preparations. Methods. Nodule bacteria sensitivity to the fungicides was studied by the method of wells in the agar medium plates with lawn seed nodule bacteria according to conventional microbiological methods. 80 μl of sterile tap water were placed into the center well of agar plate and used as control. Results. The studied B. japonicum nodule bacteria strains have different sensitivity to the single and double recommended norm of active substances of Standak Top, Fever, Akanto Plus and Maxim XL preparations with fungicidal effect. B. japonicum 634b, 646, 631, 71m, 48, 532C, 191, PC07, PC08, PC09, PC10, PC11 strains were evaluated as low-sensitive to single recommended dose of Benorad, however, the double dose of Benorad is toxic for most of the strains, except B. japonicum 71m, PC07, PC10 (low-sensitive). Conclusions. The artificial simulation of the effects of single and double recommended norm of Maxim XL, Standak Top, Fever, Akanto Plus and Benorad fungicides on nodule bacteria made it possible to determine their degree of resistance to these preparation and to identify the most resistant rhizobia in order to further use them in technology of soybean growing.
Keywords: nodule bacteria, Bradyrhizobium japonicum, fungicides, Maxim XL, Standack Top, Fever, Acanto Plus, Benorad.
Full text (PDF, in Ukrainian)
- Berbenets OV. [World-wide production of soya as an inexhaustible source of vegetable proteins and Ukraine’s place in the global trading market]. Agrosvit. 2019; 10:41–45. Ukrainian. https://doi.org/10.32702/2306-6792.2019.10.41
- Ukrainian Agrarian Confederation. UAC research [An inexhaustible source of vegetable proteins – soybeans], 2017. http://agroconf.org/content/nevicherpnedzherelo-roslinnih-bilkiv-soya
- Sichkar VI. [Pesticides and nitrogen fixation of legume crops]. Propozytsiya. Modern agro technologies for the use of biological products and growth regulators. 2015; 32–34. Ukrainian.
- Chabanyuk Ya, Brovko IY. [Factors of existence symbiosis of B. japonicum-soybean]. Propozytsiya. 2017; 3:36–37. Ukrainian.
- Kots SYa, Vorobey NA, Kyrychenko OV, Melnykova NN, Mykhalkiv LM, Pukhtayevych PP. [Microbiological preparations for agriculture]. Institute of Plant Physiology and Genetics NAS of Ukraine. Kyiv: Logos; 2016. Ukrainian.
- Gryshchuk OO, Kots SYa, Volkogon MV. [The dynamics of cytokinins content in roots and on the early stages of legume-rhizobium simbiosis forming]. Plant Physiology and biochemistry of cultivated plants genetics. 2013; 45(1):20–28. Ukrainian.
- Sichkar VI, Khukhlayev II, Bushulyan OV, Didovich SV, Koblay SV, Lavrova GD, Ganzhelo OI. [The intensification of nitrogen fixation potential in legume crops via complementary selection of hosts and microsymbionts]. Scientific Issue Ternopil Volodymyr Gnatiuk National Pedagogical University. Series: Biology. 2014; 3(60):165–169. Ukrainian.
- Volkogon VV, Nadkernychna OV, Kovalevska TM. [Microbial preparations in agriculture. Theory and Practice]. Kyiv: Agrarna nauka; 2006. Ukrainian.
- Kots SYa, Mamenko TP, Khomenko YuO. The content of hydrogen peroxide and catalase activity in different on effectiveness of symbiotic systems Glycine max – Bradyrhizobium japonicum under drought conditions. Microbiol Z. 2019; 81(4):62–75. https://doi.org/10.15407/microbiolj81.04.062
- Fox JE, Gulledge J, Engelhaupt E, Burow ME, McLachlan JA. Pecticides reduce symbiotic efficiency of nitrogen-fixing rhizobia and host plants. Proceedings of the National Academy of Sciences. 2007; 104(24):10282–10287. https://doi.org/10.1073/pnas.0611710104
- Pavlyshche AV, Yakimchyk RA, Omelchyk SV, Zhemioda AV, Kots SYa. [Symbiotic properties and seed productivity of soybean in field conditions under various methods of seed treatment with fungicides]. Plant physiology and genetics, 50(4):358–368. Ukrainian. https://doi.org/10.15407/frg2018.04.358
- Saenko GM, Bushneva NA. [Compatibility of fungicidal dressers of soybean with inoculants]. Oil crops. Scientific and technical bulletin of VNIIMK. 2018; 3(175):124–127. Russian. https://doi.org/10.25230/2412-608X-2018-3-175-124-127
- Tikhonovich IA. [Meaning of symbiogenetics for biological education]. Ecological genetics. 2007; 5(1):8–17. Russian. https://doi.org/10.17816/ecogen518-17
- Alam S, Kumar A, Kumar A, Prasad S, Tiwari A, Srivastava D, Srivastava S, Tiwari P, Singh J, Mathur B. Isolation and characterization of pesticide tolerant bacteria from brinjal rhizosphere. Int J Curr Microbiol App Sci. 2018; 7:4849–4859.
- Drouin P, Sellami M, Prevost D, Fortin J, Antoun H. Tolerance to agricultural pesticides of strains belonging to four genera of Rhizobiaceae. Journal of Environmental Science and Health. Part B. 2010; 45(8):780–788. https://doi.org/10.1080/03601234.2010.515168
- Deshmukh VV, Raut BT, Mane SS, Ingle RW, Josh MS. Compatibility of Bradyrhizobium japonicum isolates with agrochemicals. American International Journal of Research in Formal, Applied & Natural Sciences. 2014; 6(1):55–62.
- Moawad H, Abd El-Rahim WM, Shawky H, Higazy AM, Daw ZY. Evidence of fungicides degradation by rhizobia. Agricultural Sciences. 2014; 5(7):618–624. https://doi.org/10.4236/as.2014.57065
- McGuinness M, Dowling D. Plant-associated bacterial degradation of toxic organic compounds in soil. Int J Environ Res Public Health. 2009; 6(8):2226–2247. https://doi.org/10.3390/ijerph6082226
- Yakimenko MV, Begun SA, Sorokina AI. [Compatibility of collection strains of soybean rhizobia with fungicides and growth stimulants]. Far East Agrarian Bulletin. 2016; 2(38):38–41. Russian.
- Kovalevskaya TM, Gorban VP, Nadkernichnaya EV, Bardakov AG. [The effect of fundazol and rhizotorfin on efficiency of symbiosis of nodule bacteria with lupine]. Agricultural Microbiology. 2005; 1–2:52–59. Ukrainian.
- Mishra G, Kumar N, Giri K, Pandey S. In vitro interaction between fungicides and beneficial plant growth promoting Rhizobacteria. Afr J Agric Res. 2013; 8(45):5630–5633.
- Vozniuk SV, Tytova LV, Ratushinska OV, Iutynska GO. [Formation and functioning of symbiotic system and rhizosphere microbiocenosis of soybean under various fungicides application]. Mikrobiol Z. 2016; 78(4):59–70. Ukrainian. https://doi.org/10.15407/microbiolj78.04.059
- Ahmed M, Elesheikh EAE, Mahdi AA. The in vitro compatibility of some Rhizobium and Bradyrhizobium strains with fungicides. Arr Crop Sci Conf Proc. 2007; 8:1171–1178.
- Kosulnikov YuV, Laktionov YuV. [Factors which influence toxicity of legume seed disinfectants towards biological based on symbiotic nitrogen fixers]. Agricultural biology. 2018; 53(5):1037–1044. Russian. https://doi.org/10.15389/agrobiology.2018.5.1037eng
- Kozhemyakov AP, Laktionov YuV, Popova TA, Orlova AG, Kokorina AL, Vaishlya OB, Agafonov EV, Guzhvin SA, Churakov AA, Yakovleva MT. The scientific basis for the creation of new forms of microbial biochemicals. Agricultural biology. 2015; 50(3):369–376. Russian. https://doi.org/10.15389/agrobiology.2015.3.369eng
- Netrusov AI, Egorova MA, Zakharchuk LM, et al. [Practice on microbiology]. In: Netrusov AI, editor. Moscow: Akademiya; 2005. Russian.
- Alekseyev OO, Patyka VP, Gnatiuk TT. [The relationship between Bradyrhizobium japonicum and soybean bacteriosis pathogens and their sensitivity to pesticides]. Young scientist. 2016; 12.1(40):50–63. Ukrainian.
- Yaschuk VU, Ivanov DV, Krivosheya RM, Tsibulnyak YuO, Koretskiy AP. [The list of pesticides and agrochemicals permitted for use in Ukraine]. Kyiv: Yunivest Media; 2018. Ukrainian.
- Tokmakova LM, Tararyko YuO, Trepach AO, Lepekha OP, Larchenko IV. [The influence of modern seed protectants of agricultural crops on viability and functional activity of biological agents of microbial preparations]. Agricultural Microbiology. 2013; 18:120–131. Ukrainian.
- Ahemad M, Khan MS. Productivity of greengram in tebuconazole-stressed soil, by using a tolerant and plant growth-promoting Bradyrhizobium sp. MRM6 strain. Acta physiol plant. 2012; 34(1):245–254. https://doi.org/10.1007/s11738-011-0823-8