Mikrobiol. Z. 2019; 81(6):30-44. Russian.
Microbiome of Soybean Rhizosphere under Fungicides and Complex Inoculation Application
Vozniuk S.V.1, Tytova L.V.1, Pinaev A.G.2, Andronov E.E.2, Iutynska G.O.1
1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
2All-Russian Research Institute for Agricultural Microbiology
3 Podbel’skogo Str., St. Petersburg, 196608, RF
The biodiversity of the rhizosphere microbiota plays a key role for improving the productivity of cultural plants, but the soybean rhizosphere microbiome has not been studied sufficiently under various pre-sowing seed treatments. Aim. To investigate soybean rhizosphere microbiome under the pre-sowing treatment of seeds with the fungicides followed by inoculation with the complex microbial bioformulation Ecovital. Methods. Physiological (vegetation experiments), microbiological (inoculant production), molecular biological (high-performance sequencing). Results. In the microbiome of the soybean rhizosphere in dark gray podzolic soil, 23 phyla (including 2 archea), 63 classes and unidentified sequences (7.1–8.3%) were found. The dominants, with a representation from 1% to 26.6%, were 12 phyla: Acidobacteria, Actinobacteria, Bacteroidetes, Cyanjbacteria, Chloroflexi, Firmicutes, Gemmatimonadetes, Planctomycetes, Proteobacteria, TM7, Verrucomicrobia and Crenarchaeota. The representation of dominant phyla Acidobacteria, Firmicutes, Gemmatimonadetes and Rhizobiales increased under complex treatment of seeds compared to the control variant. The sequences related to the Phyllobacterium and Methylobacterium genera, were found only in the variants with using of fungicides and Ecovital. Conclusions. The combined pre-sowing treatment of soybean seeds did not affect the qualitative composition of the dominants, but change the representation of individual taxa. The nodule bacteria of the Bradyhizobium genus were not detected in the rhizosphere soil of the control variant but under combined treatment of seeds their relative representation did not exceed 0.1%. It indicates the need of highly effective rhizobia strains application for soybean cultivation.
Keywords: soybean, rhizosphere, microbiome, inoculation, fungicides, high performance sequencing, biodiversity.
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