Mikrobiol. Z. 2019; 81(6):3-15. Ukrainian.
Effect of Complex Endophytic-Rhizobial Inoculation on Rhizosphere Microbiota
and Productivity of Soybean
Iutynska H.O.1, Goloborodko S.P.2, Tytova L.V.1, Dubinska O.D.2
1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
2Institute of Irrigated Agriculture, NAAS of Ukraine
Naddnipryanske, Kherson, 73483, Ukraine
Modern studies of the microbiome of leguminous plants have revealed nodule bacteria and non-rhizobial prokaryotic endophytes, which inhabit the tissues of various organs. The interactions of symbiotic and endophytic bacteria, as well as their joint effect on the plant are not well studied. Аim. Тo study the rhizosphere microbiota of soybean and its productivity under complex inoculation by nodule bacteria together with non-rhizobial endophytic bacteria isolated from soybean nodules. Methods. Field experiments were conducted in the Kherson region under irrigation conditions in the fields of the Askanian Experimental Station of the Institute of Irrigated Agriculture of the National Academy of Agrarian Sciences of Ukraine. Seeds of soybean ultra-fast mature cultivar Diona and middle-mature cultivar Aratta were inoculated with RhizobinK bioformulation based on the association of nodule bacteria strains Bradyrhizobium japonicum UCM B-6018, B-6023 and B-6035, and also with complex of RhizobinK with B. megaterium B-5724, or with one of the endophytic bacteria strain: Paenibacillus sp.1, Bacillus cereus 4, Brevibacillus sp.5, Pseudomonas brassicacearum 6. The number of microorganisms of the main ecologicaltrophic groups in the rhizosphere of soybean was determined using the method of sowing the soil suspension on nutrient agar media. Results. The number of pedotrophic, phosphatemobilizing, oligoazotrophic and nitrogen-fixing bacteria was increased in the soybean rhizosphere soil of Diona and Aratta cultivars after inoculation with RhizobinK alone or together with one of the endophytes ‒ B. cereus 4, Brevibacillus sp.5, P. brassicacearum 6. Endophytic-rhizobial inoculation increases the number of beans and seeds on the plant. The highest yield was obtained after complex inoculation of soybean seeds of Diona and Aratta cultivars with RizobinK and B. cereus 4: 3.12 and 2.55 t/ha, respectively, which exceeded the parameters of the control variants by 26.6% and 16.5% respectively with simultaneous increasing of protein and fat in seeds. Conclusions. Research of complex effect of nodule and endophytic bacteria on plants will provide an opportunity to develop a strategy to increase the productivity of legumes based on new endophytic-rhizobial inoculants.
Keywords: rhizobia, endophytes, rhizosphere, soybean, seed productivity, yield quality.
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- [Bioregulation of microbial-plant systems]. Iutynska GO, Ponomarenko SP, editors. Kyiv: Nichlava, 2010. Russian.
- Brovko IS, Tytova LV, Iutynska HO. [Influence of endophytic soybean bacteria on the rhizobium-soybean symbiosis and rhizosphere microbial community]. Microbiology and Biotechnology. 2015; 4:36–45. Ukrainian. https://doi.org/10.18524/2307-4663.2015.4(32).57460
- Cohen AC, Travaglia CN, Bottini R, Piccoli PN. Participation of abscisic acid and gibberellins producedby endophytic Azospirillum in the alleviation of droughte effects in maize. Botany. 2009; 87(5):455–462. https://doi.org/10.1139/B09-023
- Compant S, Clement C, Sessitsch A. Plant growth-promoting bacteria in the rhizo- and endosphere of plants: their role, colonization, mechanisms involved and prospects for utilization. Soil Biol Biochem. 2010; 42:669–678. https://doi.org/10.1016/j.soilbio.2009.11.024
- Dudeja SS1, Giri R, Saini R, Suneja-Madan P, Kothe E. Interaction of endophytic microbes with legumes. Journal of Basic Microbiology. 2012; 52(3):248–260. https://doi.org/10.1002/jobm.201100063
- Grover M, Madhubala R, Ali SZ, Yadav SK, Venkateswarlu B. Influence of Bacillus spp. strains on seedling growth and physiological parameters of sorghum undermoisture stress conditions. Journal of Basic Microbiology. 2014; 54(9):951–961. https://doi.org/10.1002/jobm.201300250
- Iutynska GO, Biliavska LO, Tytova LV, et al. [Application of new bioformulations for plant growing. Methodical recommendations]. Kyiv; 2018. Ukrainian.
- Kurdish IK. [Introduction of microorganisms in agroecosystems]. Kyiv: Naukova dumka; 2010. Ukrainian.
- Le Cocq K, Gurr SJ, Hirsch PR, Mauchline TH. Exploitation of endophytes for sustainable agricultural intensification. Mol Plant Pathol. 2017; 18:469–73. https://doi.org/10.1111/mpp.12483
- Mei C, Flinn BS. The use of beneficial microbial endophytes for plant biomass and stress tolerance improvement. Recent Patents on Biotechnology. 2010; 4(1):81–95. https://doi.org/10.2174/187220810790069523
- [Microbial preparations in agriculture. Theory and practice]. Volkohon VV, editor. Kyiv: Agrarian science, 2006. Ukrainian.
- Miliute I, Buzaite O, Baniulis D, Stanys V. Bacterial endophytes in agricultural crops and their role in stress tolerance. Zemdirbyste-Agriculture. 2015; 102 (4): 465–478. https://doi.org/10.13080/z-a.2015.102.060
- Naveed M, Aziz MZ, Yaseen M. Perspectives of Using Endophytic Microbes for Legume Improvement. In: Microbes for Legume Improvement. Springer. Almas Zaidi M, Saghir K, Javed M, editors. 2017. p. 277–299. https://doi.org/10.1007/978-3-319-59174-2_12
- O’Callaghan M. Microbial inoculation of seed for improved crop performance: issues and opportunities. Appl Microbiol Biotechnol. 2016; 100(13):5729–5746. https://doi.org/10.1007/s00253-016-7590-9
- Pandey PK, Singh S, Singh AK, Ramkrishna S, Raj Narain SY, M. Chandrakumar S. Inside the plant: Bacterial endophytes and abiotic stress alleviation. Journal of Applied and Natural Science. 2016; 8(4):1899–1904. https://doi.org/10.31018/jans.v8i4.1059
- Saini R, Kumar C, Dudeja SS, Pathak DV Beneficial effects of inoculation of endophytic bacterial isolates from roots and nodules in chickpea. Int J Curr Microbiol App Sci 2015; 4(10):207–221.
- Saravanakumar D, Kavino M, Raguchander T, Subbian P, Samiyappan R. Plant growth promoting bacteria enhance water stress resistance in green gram plants. Acta Physiologiae Plantarum. 2011; 33(1):203–209. https://doi.org/10.1007/s11738-010-0539-1
- Stajkovic O, Delic D, Josic D, Kuzmanovic D, Rasulic N, Knezevic-Vukcevic J. Improvement of common bean growth by co-inoculation with Rhizobium and plant growth promoting bacteria. Romanian Biotechnological Letters. 2011; 16(1):5919–5926.
- Sturz, AV, Christie BR, Matheson BG, Nowak J. Biodiversity of endophytic bacteria which colonize red clover nodules, roots, stems and foliage and their influence on host growth. Biol Fertil Soils, 1997; 25:13–19. https://doi.org/10.1007/s003740050273
- Taurian T, Ibáñez F, Angelini J, Tonelli ML, Fabr A. Endophytic Bacteria and Their Role in Legumes Growth Promotion. Bacteria in Agrobiology. In: Plant Probiotics. Springer, Dinesh K. Maheshwari, editor. 2012. p. 141–168. https://doi.org/10.1007/978-3-642-27515-9_8
- Timmusk S, El-Daim IA, Copolovici L, Tanilas T, Kännaste A, Behers L, Niinemets Ü. Drought-tolerance of wheat improved by rhizosphere bacteria from harsh environments: enhanced biomass production and reduced emissions of stress volatiles. PloS One. 2014; 9(5):e96086. https://doi.org/10.1371/journal.pone.0096086
- Trivedi G, Shah R, Patel P, Saraf M. Role of Endophytes in Agricultural Crops Under Drought Stress: Current and Future Prospects. Journal of Applied Microbiology. 2017; 3(4):174–188.
- Vigani G, Rolli E, Marasco R, Dell’Orto M, Michoud G, Soussi A, Raddadi N, Borin S, Sorlini C, Zocchi G, Daffonchio D. Root bacterial endophytes confer drought resistance and enhance expression and activity of a vacuolar H+-pumping pyrophosphatase in pepper plants. Environ Microbiol. 2018. 22. https://doi.org/10.1111/1462-2920.14272
- Zvyagintsev DH, Alilyeva IV, Babieva IP, Byzov BA, et al. [Methods of soil microbiology and biochemistry]. In: Zvyagintsev DH, editor. Moscow: Publishing house of the Moscow University; 1991. Russian.