Mikrobiol. Z. 2019; 81(2):41-50. Russian.
Homology of Primary Structures of the Beta-Galactosidase Gene of Streptomyces globisporus 1912
and Similar Genes of Streptomycetes
Polishchuk L.V., Bambura O.I.
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
The purpose of this study is to determine the degree of homology of nucleotide sequences of the β-galactosidase genes of a number of streptomycetes strains and S. globisporus 1912 gene encoding β-galactosidase. The genomes of 30 streptomycetes strains with different degrees of taxonomic relationship to S. globisporus 1912 were analyzed. Methods. Alignment of the primary structures of genomes of 31 streptomycetes from the “Nucletide collection” and “Whole-genome shotgun contigs” databases on the NCBI server was done using blastn and bl2seq programs from the BLAST package. Results. Alignment revealed a significant degree of homology (from 66% to 97%) of the sequences of beta-galactosidase genes of the majority (90%) of the studied streptomycete strains to the similar S. globisporus 1912-4Сrt gene. Only primary structures of the beta-galactosidase genes of strains – members of S. albovinaceus subclades had a degree of homology of their structures to the reference gene above 95.5%. It was established that up to 6 beta-galactosidase genes are presented in the genomes of the strains from the presented random sample. However, in the genomes most strains (51.6%) are present only one beta-galactosidase gene per chromosome, and in 3 of them, the structures of the beta-galactosidase genes have no homology with the reference gene structure of S. globisporus 1912-4Сrt. Conclusions. Homology of the primary structures of beta-galactosidase genes of streptomycetes strains and the similar gene of S. globisporus 1912-4Crt depends on their genetic affinity for the strain S. globisporus 1912. As an example, the primary structures of beta-galactosidase genes of strains from S. albovinaceus subclades were most identical to the structures of the reference gene (above 95.5%).
Keywords: streptomycete, β-galactosidase gene, homology, overlap, genome, clade, alignment, primary structure.
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