Mikrobiol. Z. 2018; 80(6):15-27.
Depositary Function of Pseudomonas aeruginosa Biofilm on Media
with Different Carbon Source Concentration
Balko O.I., Avdeeva L.V., Balko O.B.
Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
The aim of work was study of peculiarities of Pseudomonas aeruginosa biofilm formation on media with different concentration of glucose as sole source of carbon and energy. Methods. Three Pseudomonas aeruginosa strains were cultivated in stationary system on glass in Kozer medium with 0.1 %, 5 % and 20 % of glucose. The effect of unfavourable conditions on microorganism growth was analyzed by intensity of biofilm formation and quantities of viable cells in biofilm and planktonic form. Results. It was determined that investigated cultures maintained the ability to grow and form biofilm in the presence of 0.1 - 20 % glucose in Kozer medium. If there was 0.1 % of glucose, dynamics of biofilm accumulation was characterized by strain peculiarities. But under increase of carbon source concentration to 20 %, these differences became less evident and biofilm formation acquired common for all used cultures regularities. The increase of glucose concentration caused reduction of the sample area covered by biofilm and fall of microorganism quantity in its composition, stabilized the biofilm formation dynamics, but didn’t influence on bacterium titre in planktonic form. Conclusions. Under the influence of unfavourable conditions, besides protective function, Pseudomonas aeruginosa biofilm can perform the role of depositary formation, maintaining stable cell quantity in planktonic form.
Keywords: Pseudomonas aeruginosa, biofilm formation, microorganisms in planktonic and biofilm form, carbon source concentration.
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