Mikrobiol. Z. 2018; 80(2):14-27. Ukrainian.
Antimicrobial and Anti-adhesive Activity of Surfactants Synthesized
by Acinetobacter calcoaceticus IMV B-7241 on Technical Glycerol
Pirog T.P.1,2, Lutsai D.A.1, Shevchuk T.A.2, Iutynska G.O.2, Elperin I.V.1
1National University of Food Technologies
68 Volodymyrska Str., Kyiv, 01601, Ukraine
2Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
Aim. Comparison of biological properties (antimicrobial and anti-adhesive activity, including role in bioflms destruction) of surfactants Acinetobacter calcoaceticus IMV B-7241, synthesized on purifed and technical glycerol (waste of biodiesel production). Methods. The surfactants were extracted from supernatant of cultural liquid with a mixture of chloroform and methanol (2 : 1). The number of adherent cells and the degree of bioflm destruction were determined by the spectrophotometric method, the antimicrobial properties of the surfactants were analyzed by the minimum inhibitory concentration. Results. The antimicrobial activity of A. calcoaceticus IMV B-7241 surfactants synthesized on technical glycerol was 1 − 2 orders lower, adhesion of test cultures on abiotic materials treated with such preparations was 1.2 − 4 times lower and degree of bacterial bioflms destruction − in 1.3 − 4 times less in comparison with the parameters established for surfactants, obtained on medium with equimolar by carbon concentration of purifed glycerol. The decreasing antimicrobial and anti-adhesive activity of the surfactants may be due to the inhibitory efect of K+ and Na+ excess concentration (components of technical glycerol) on the activity of NADP+-dependent glutamate dehydrogenase: in the presence of 50 ‒ 100 mM of potassium and sodium cations enzymatic activity decreased in 1.2−1.8 times. Conclusions. Despite the lower antimicrobial and anti-adhesive activity of surfactants synthesized by A. calcoaceticus IMV B-7241 on waste of biodiesel production, these values (minimum inhibitory concentrationі 0.96 − 15.2 μg/ml, degree of bioflms destruction by 50 % at surfactants concentration of 124 μg/ml) are comparable with those established for known microbial surface-active aminolipids.
Keywords: Acinetobacter calcoaceticus ІМВ В-7241, surfactants, biological properties, waste of biodiesel production.
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