Mikrobiol. Z. 2018; 80(1):15-26. Russian.
Intensification of Acinetobacter calcoaceticus IMV B-7241 Surfactants
Synthesis on Waste Sunflower Oil
Pirog T.P.1,2, Nikituk L.V.1, Antonuk S.I.1, Shevchuk T.A.2, Iutynskaya G.A.2
1National University of Food Technologies
68 Volodymyrska Str., Kyiv, 01601, Ukraine
2Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
Aim. To establish cultivation conditions of Acinetobacter calcoaceticus IMV B-7241 on waste (refried) sunflower oil of various quality, which provide the maximal indicators of surfactants synthesis. Methods. A. calcoaceticus IMV B-7241 was grown in liquid medium containing 2 − 6 % oil after frying the potato and meat including in the presence of biosynthesis precursors (glucose, organic acids). The amount of synthesized surfactants was determined by the weight method after extraction from the supernatant of culture liquid with a mixture of chloroform and methanol (2 : 1), emulsifying properties according to emulsification index of culture liquid using sunflower oil as a substrate. Results. The highest indices of surfactant synthesis on all substrates were observed with using the inoculum grown on the corresponding fried oil. The maximum concentration of surfactants (8.5 ± 0.42 and 7.9 ± 0.39 g/l) was achieved under cultivation of IMV B-7241 strain on oil after frying meat (4 %) and potato selyanski (6 %) respectively. When the biosynthesis precursors (glucose, 0.1 %, potassium fumarate, 0.01 − 0.1% and potassium citrate, 0.01 − 0.1 %) were added into medium with 2 % of the fried oil, the surfactants concentration was increased by 2.3 − 3.4 times in comparison with the indices on medium without glucose and organic acids. Addition of fumarate and citrate in form of sodium salts into the medium of IMB B-7241 strain cultivation was not accompanied by increasing surfactants synthesis due to inhibitory effect of sodium cations on activity of enzymes of anaplerotic reaction (phosphoenolpyruvate-(PEP)-carboxylase) and surfactants biosynthesis (PEP-carboxykinase, NADP+-dependent glutamate dehydrogenase). Conclusions. A doubling of waste oil and urea concentration in cultivation medium of IMV B-7241 strain (up to 4 % and 0.7 g/l, respectively) and replacing refined oil in medium for inoculum obtaining by waste was accompanied by increasing amount of synthesized surfactants in 4 − 5 folds in comparison with those on the base medium. The using fried oils for surfactants biosynthesis will allow not only to reduce the cost of final product, but also to utilize toxic waste, emissions of which into the environment in Ukraine are not regulated.
Keywords: Acinetobacter calcoaceticus IMV B-7241, surfactants, fried sunflower oil, precursors of biosynthesis, intensification of biosynthesis.
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