Mikrobiol. Z. 2017; 79(4):21-29. Ukrainian.
The Role of Acinetobacter calcoaceticus IMV В-7241 Surfactants in Biofilms Destruction
Pirog T.P.1,2, Savenko I.V.1, Lutsai D.A.1, Shevchuk T.A.2, Iutynska G.O.2
1National University of Food Technologies
68 Volodymyrska Str., Kyiv, 01601, Ukraine
2Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
Aim. To study the ability of surface-active agents (surfactants) synthesized Acinetobacter calcoaceticus IMV B-7241 on various substrates (ethanol, glycerol, n-hexadecane), destroy a bioflm certain bacteria and yeasts. Methods. Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2 : 1). The degree of bioflm destruction was determined as difference between the number of adhered cells in untreated and treated with surfactant holes of polystyrene immunological plate containing pre-formed bioflm of test cultures and was expressed as a percentage. The number of adherent cells was determined by spectrophotometrically. Results. The dependence of degree of bioflms destruction in the presence of A. calcoaceticus IMV B-7241 surfactants on the nature of carbon source in medium of producer cultivation, concentration of surfactants in the preparations and test culture type was established. Degree of Escherichia coli ІЕМ-1 bioflm destruction in the presence of surfactants synthesized on all tested substrates practically did not differ and was 25 − 53 % depending on the concentration of the surfactants (0,04 − 1,28 mg/ml). Bacillus subtilis BT-2 bioflm destruction reached 71 − 86 % after addition of surfactants (0,16 − 0,64 mg/ml) obtained on ethanol and glycerol. Maximum destruction of Staphylococcus aureus BMS-1 bioflm (69 − 88 %) and Candida albicans D-6 (42 %) was observed in the presence of surfactants (0,32 − 1,28 mg/ml) synthesized on n-hexadecane. Conclusions. The effective concentrations of A. calcoaceticus IMV B-7241 surfactants, providing a high degree of bacterial bioflms destruction are comparable to those of the known microbial surfactants. The obtained data indicates the need for research the infuence of producer cultivation conditions on the biological properties of fnal product.
Key words: Acinetobacter calcoaceticus ІМV В-7241, surfactants, destruction of the bioflm, cultivation conditionsa.
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- Santos D.K., Rufno R.D., Luna J.M., Santos V.A., Sarubbo L.A. Biosurfactants: multifunctional biomolecules of the 21st century. Int. J. Mol. Sci. 2016; 17(3).
- Cawoy H., Debois D., Franzil L., De Pauw E., Thonart P., Ongena M. Lipopeptides as main ingredients for inhibition of fungal phytopathogens by subtilis/amyloliquefaciens. Microb. Biotechnol. 2015; 8(2):281−295.
- Meena K.R., Kanwar S.S. Lipopeptides as the antifungal and antibacterial agents: applications in food safety and therapeutics. Biomed. Res. Int. 2015.
- Padmapriya B., Suganthi S. Antimicrobial and anti adhesive activity of purifed biosurfactants produced by Candida species. Middle-East J. Sci. Res. 2013; 14(10): 1359–1369.
- Sriram M.I., Kalishwaralal K., Deepak V, Gracerosepat R., Srisakthi K., Gurunathan S. Bioflm inhibition and antimicrobial action of lipopeptide biosurfactant produced by heavy metal tolerant strain Bacillus cereus NK1. Colloids Surf. B. Biointerfaces. 2011; 85(2): 174–181. https://doi.org/10.1016/j.colsurfb.2011.02.026
- Turbhekar R., Malik N., Dey D., Thakare D. Disruption of Candida albicans bioflms by rhamnolipid obtained from Pseudomonas aeruginosa RT. IJRSB. 2015; 3(3):73–78.
- Vilela S.F., Barbosa J.O., Rossoni R.D., Santos J.D., Prata M.C., Anbinder A.L., Jorge A.O., Junqueira J.C. Lactobacillus acidophilus ATCC 4356 inhibits bioflm formation by C. albicans and attenuates the experimental candidiasis in Galleria mellonella. Virulence. 2015; 6(1):29–39. https://doi.org/10.4161/21505594.2014.981486
- Jolly M.J. Inhibitory effect of biosurfactant purifed from probiotic yeast against bioflm producers. IOSR-JESTFT. 2013; 6(1):51–55. https://doi.org/10.9790/2402-0615155
- Pirog T.P., Konon A.D., Beregovaya K.A., Shulyakova M.A. Antiadhesive properties of the surfactants of Acinetobacter calcoaceticus IMB B-7241, Rhodococcus erythropolis IMB Ac-5017, and Nocardia vaccinii IMB B-7405. Microbiology. 2014, 83(6):732–739.
- Pirog T.P., Savenko I.V., Shevchuk T.A. [Effect of cultivation conditions of Acinetobacter calcoaceticus ІMV B-7241 on surfactants antiadhesive properties]. Microbiol. Zh. 2016; 78(1):2−12. Russian.
- Gomes M-Z.V., Nitschke M. Evaluation of rhamnolipids surfactants as agents to reduce the adhesion of Staphylococcus aureus to polystyrene surfaces. Lett. Appl. Microbiol. 2012; 49(1):960–965.
- Lakin, G.F. [Biometriya] (Biometry), Moscow: Vysshaya shkola, 1990. Russian.
- Gomes M-Z.V., Nitschke М. Evaluation of rhamnolipid and surfactin to reduce the adhesion and remove bioflms of individual and mixed cultures of food pathogenic bacteria. Food Control. 2012; 25(2):441−447.
- Das P., Yang X-P., Ma L.Z. Analysis of biosurfactants from industrially viable Pseudomonas strain isolated from crude oil suggests how rhamnolipids congeners affect emulsifcation property and antimicrobial activity. Front. Microbiol. 2014.
- Fracchia L., Cavallo M., Allegrone G., Martinotti M.G. A Lactobacillus – derived biosurfactant inhibits bioflm formation of human pathogenic Candida albicans bioflm producers. In: Current research, technology and education topics in applied microbiology and microbial biotechnology (Ed. Mendez-Vilas A.). Formatex Research Center. 2010, P. 827–837.
- Sambanthamoorthy K, Feng X, Patel R, Patel S, Paranavitana C. Antimicrobial and antibioflm potential of biosurfactants isolated from lactobacilli against multi-drugresistant pathogens. BMC Microbiol. 2014.