Mikrobiol. Z. 2017; 79(3):27-35. Ukrainian.
Regulation of Antimicrobial Activity of Surfactants, Synthesized by Nocardia vaccinii IMV B-7405
Pirog T.P.1,2, Nikituk L.V.1, Makienko V.O.1, Shevchuk T.A.2, Iutynska G.O.2
1National University of Food Technologies
68 Volodymyrska Str., Kyiv, 01601, Ukraine
2Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
Aim. To study the possibility of enhancement antimicrobial activity of the surfactants synthesized Nocardia vaccinii ІMV B-7405 after introduction into the medium Escherichia coli ІEM-1 and Bacillus subtilis BT-2. Methods. Live and inactivated cells of bacteria inductors (E. coli ІEM-1 and B. subtilis BT-2) were introduced into cultivation medium of surfactant producer at the beginning of the process and in the exponential growth phase. Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2 : 1). Antimicrobial against bacteria and yeast properties of the surfactant was determined by index of the minimum inhibitory concentration (MIC). Results. It has been established that introduction into cultivation medium of N. vaccinii ІMV B-7405 both live and inactivated E. coli ІEM-1 and B. subtilis BT-2 cells was accompanied by synthesis of surfactant with improved antimicrobial activity. The minimum inhibitory concentration against bacteria (E. coli ІЕМ-1, B. subtilis BТ-2, Pseudomonas sp. MІ-2, Proteus vulgaris PА-12) and yeast Candida albicans D-6 of surfactants synthesized in the presence of E. coli ІEM-1 and B. subtilis BT-2 were 6 – 50 μg/ml, that in 2.4 - 13 times lower than MIC of surfactant obtained in the medium without bacteria inductors. Conclusions. The data obtained indicate the possibility of regulation of antimicrobial activity of N. vaccinii ІMV B-7405 surfactants when cells of other bacteria including E. coli ІEM-1 and B. subtilis BT-2 were introduced into cultivation medium of surfactant producer.
Key words: Nocardia vaccinii ІMV B-7405, surfactants, improved antimicrobial activity, bacteria inductors.
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