Mikrobiol. Z. 2017; 79(2):23-32. Russian.
doi: https://doi.org/10.15407/microbiolj79.02.023

Functioning of Sulfidogenic Microbial Community, Including Transcojugants
with the Rp4 and R68.45 Plasmids

Abdulina D.R., Purish L.M., Iutynska G.O.

Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine

Aim of the study is to evaluate the infuence of the extrachromosomal genetic elements (i.e. plasmids) on the morphological and corrosion features of the sulfdogenic microbial communities during the bioflm formation on steel surface. Мethods. Microbiological, molecular biological. Results. Bacteria from sulfdogenic microbial community harboring RP4 and R68.45 plasmids changed their colony morphotype: the transconjugated bacteria formed the colonies of the S-, O-types instead the R-type colonies of the wild strains. The changes in the physiological and corrosive activity of the transconjugated bacteria and sulfdogenic communities were demonstrated. Under the bioflm formation on the steel surface by the sulfdogenic microbial community including Desulfovibrio sp. 10, Bacillus subtilis 36 and transconjugated strain Pseudomonas aeruginosa 27 (R68.45) the protein synthesis in the bioflm was decreased in 2,3 - 4,4 times, the hydrogen sulfde production – 1,5 times and corrosion rate inhibition –  2 times in comparison with non-plasmid harboring community. Conclusions. The study of the infuence of the extrachromosomal genetic elements during bioflm formation by the sulfdogenic microbial community may help to create new approaches for corrosion prevention and develop alternative biologically based anticorrosion measures for metals corrosion protection.

Key words: sulfdogenic microbial community, transconjugants, corrosive activity.

Full text (PDF, in Russian)

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