Mikrobiol. Z. 2016; 78(4):59-70. Ukrainian.
Formation and Functioning of Symbiotic Systems and Rhizosphere Microbiocenisis
of Soybean under Various Fungicides Application
Vozniuk S.V.1, Tytova L.V.1, Ratushinska O.V.2, Iutynska G.O.1
1Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
2Taras Shevchenko National University of Kyiv
2 Acad. Hlushkov Av., Kyiv, 03022, Ukraine
The Relevance. At the recent years in soybean crops the quantity of plant pathogenic fungi has increased. The fungicides of systemic and contact action have been applicated intensively against of them. After introducing into the soil fungicides and/or their degradation products can to disrupt the activities of non-target objects – beneficial soil microorganisms, inhibit nodulation process and the nitrogen-fixing activity of diazotrophs. The purpose of the work was to investigate the impact of combined application of fungicides with inoculation on the soybean symbiotic system and rhizosphere microorganisms. The Methods. The microbiological and statistical methods, gas chromatography method. The Results. Inoculation of seeds by the highly active Bradyrhizobium japonicum UCM B-6035, UCM B-6018 and UCM B-6023 strains the activity of nitrogen-fixing symbiotic systems increased by 1.4 - 3.4 times in comparison with the variant without of fungicides application and bacterization. Seed treatment by Vitavaks 200 FF fungicide caused a decrease of nitrogen-fixing activity of rhizobia industrial strains in symbiosis with soybean by 3 - 5 times. The seeds inoculation by B. japonicum UCM B-6035 strain promoted to reduce the negative impact of the Maxim Star 025 FS fungicide on the nitrogenase activity of nodulation apparatus. The positive effect of seeds bacterization was observed in the increase of the quantity of rhizosphere microorganisms of main ecological trophic groups. In the variant with seed treatment by Maxim Star 025 FS and Kinto duo fungicides was found a decrease in the number of microorganisms of studied groups in comparison with the control variant; the Vitavaks 200 FF fungicide application promoted to improve of these microorganisms development compared with the variant without the fungicides application and bacterization. At the inoculation of rhizobia industrial strains the negative effect of the Maxim Star 025 FS and Kinto duo fungicides to oligoazotrophic and prototrophic microorganisms was not observed. The Conclusion. The symbiotic system of variant with the combined application of the Kinto duo fungicide with B. japonicum UCM B-6023 strain characterized by the highest nodulation and nitrogen-fixing activity.
Key words: fungicides, inoculation, soybean, rhizobia, symbiosis, nodulation process, nitrogen-fixing activity, rhizosphere, microbiocenosis.
Full text (PDF, in Ukrainian)
- Ahmed M., Elesheikh E.A.E, Mahdi A.A. The in vitro compatibility of some Rhizobium and Bradyrhizobium strains with fungicides. Afr. Crop Sci. Conf. Proc. 2007; 8:1171–1178.
- Ahemad M., Khan M.S. Productivity of greengram in tebuconazole-stressed soil, by using a tolerant and plant growth-promoting Bradyrhizobium sp. MRM 6 strain. Acta Physiol Plant. 2012; 34:245–254. https://doi.org/10.1007/s11738-011-0823-8
- Ahmead M., Khan M.S. Pesticides as antagonists of rhizobia and the legume-rhizobium symbiosis: a paradigmatic and mechanistic outlook. Biochemistry and Molecular Biology. 2013; 1(4):63–75. https://doi.org/10.12966/bmb.12.02.2013
- Bikrol A., Saxena N., Singh K. Response of Glycine max in relation to nitrogen fixation as influenced by fungicide seed treatment. African J. Biotechnol. 2005; 4(7):667–671. https://doi.org/10.5897/AJB2005.000-3122
- Dragovoz I.V., Leonova N.O., Biliavska L.O., Yavorska V.K., Iutynska G.O. Phytohormone production by some free-living and symbiotic soil microorganisms. Reports of the National Academy of Sciences of Ukraine. 2010; 12:154–159.
- Filimon M.N., Voia S.O., Popescu R., Dumitrescu G., Ciochina P.L., Mituletu M., Vlad D.C. The effect of some insecticides on soil microorganisms based on enzymatic and bacteriological analyses. Romanian Biotechnological Letters. 2015; 20(3):10439–10447.
- Fox J.E., Gulledge J., Engelhaupt E., Burow M.E., McLachlan J.A. Pesticides reduce symbiotic efficiency of nitrogen-fixing rhizobia and host plants. PNAS. 2007; 104(24):10282–10287. https://doi.org/10.1073/pnas.0611710104
- Guene N.F.D., Diouf A., Gueye M. Nodulation and nitrogen fixation of field grown common bean (Phaseolus vulgaris) as influenced by fungicide seed treatment. Afr. J. Biotechnol. 2003; 2(7):198–201. https://doi.org/10.5897/AJB2003.000-1041
- Hardy R.W., Burns R.C., Holsten R.D. Application of the acetylene-ethylene assay for measurement of nitrogen fixation. Soil. Biol. Biochem. 1973; 5(1):41–83. https://doi.org/10.1016/0038-0717(73)90093-X
- Iutynska G.O., Tytova L.V., Leonova N.O., Antypchuk A.F., Brovko I.C. Complex bacterial preparations. Bioregulation of microbial-plant system. Editors G.O. Iutynska, S.P. Ponomarenko. Kyiv: Nichlava, 2010. P. 352–376.
- Kaur C., Maini P., Shukla N.P. Interaction studies of copper fungicides with biological environment of soil. Current World Environ. 2007; 2(1):89–92. https://doi.org/10.12944/CWE.2.1.19
- Medić-Pap S., Milošević M., Jasnić S. Soybean seed-borne fungi in the Vojvodina province. Phytopathol. Pol. 2007; 45:55–65.
- Mishra G., Kumar N., Giri K., Pandey S. In vitro interaction between fungicides and beneficial plant growth promoting Rhizobacteria. Afr. J. Agric. Res. 2013; 8(45):5630–5633.
- Mishra G., Kumar N., Giri K., Pandey S., Kumar R. Effect of fungicides and bioagents on number of microorganisms in soil and yield of soybean (Glycine max). Nusantara Bioscience. 2014; 6(1):45–48.
- Monkiedje A., Ilori M.O., Spiteller M. Soil quality changes resulting from the application of the fungicides mefenoxam and metalaxyl to a sandy loam soil. Soil Biol. Biochem. 2002; 34:1939–1948. https://doi.org/10.1016/S0038-0717(02)00211-0
- Pudełko K., Mądrzak C.J. Influence of fungicide Funaben T on nodulation of soybean (Glycine max (L.) Merr.) in the field conditions. Journal of Plant Protection Research. 2004; 44(2):155–160.
- Pat. 101388 UA, MPK C05F 11/00, C12P 39/00. Complex microbial bioformulation Ekovital for the seeds legumes inoculation. Tytova L.V., Leonova N.O., Brovko I.C., Iutynska G.O. Publ. 25.03.13. Bul. 6.
- Pat. 85089 UA, MPK C05F 11/08 C12N 1/20, C12R 1/01. Inoculant for the increase of soybean productivity. Antypchuk A.F., Tytova L.V., Leonova N.O., Iutynska G.O. Publ. 25.12.08. Bul. 24.
- Salantur A., Ozturk A., Akten S. Growth and yield response of spring wheat (Triticum aestivum L.) to inoculation with rhizobacteria. Plant Soil Enviroment. 2006; 52(3):111–118. https://doi.org/10.17221/3354-PSE
- Tepper E.Z., Shilnikova V.K., Pereverzeva G.I. Workshop on microbiology: a textbook for high schools. Moscow: Drofa, 2005.
- Tikhonovich I.A., Kojemyakov A.P., Chebotar V.K. Biopreparations in agriculture (Methodology and practice of microorganisms application in plant growing and crop production). Editor I.A. Tikhonovich. Moscow: Rosselkhozakademiia, 2005.
- Tsavkelova E.A., Klimova S.Yu., Cherdyntseva T.A., Netrusov A.I. Microbial producers of plant growth stimulators and their practical use: A review. Applied Biochemistry and Microbiology. 2006; 42(2):117–126. https://doi.org/10.1134/S0003683806020013
- Volkogon V.V., Nadkernichna O.V., Kovalevska T.M. Microbial preparations in agriculture: theory and practice. Editor V.V. Volkogon. Kyiv: Ahrarna nauka, 2006.