Mikrobiol. Z. 2016; 78(4):48-58. Russian.
Effect of Zn2+on Synthesis of Acinetobacter calcoaceticus IMV B-7241 Surfactants
with Antimicrobial and Antiadhesive Properties
Pirog T.P.1,2, Savenko I.V.1, Shevchuk T.A.2
1National University of Food Technologies
68 Volodymyrska Str., Kyiv, 01601, Ukraine
2Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154 Akad. Zabolotny Str., Kyiv, 03143, Ukraine
Aim. To study the effect of zinc cations in the composition of ethanol and n-hexadecane containing medium on the antiadhesive and antimicrobial activity of Acinetobacter calcoaceticus ІMV B-7241 surfactants. Methods. Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2 : 1). The number of attached cells was determined spectrophotometrically, antimicrobial properties – by index of the minimum inhibitory concentration (MIC). Results. Adding Zn2+ (38 mmol/l) into medium with ethanol and n-hexadecane containing copper sulphate and iron sulphate, was accompanied by the formation of surfactant with higher antimicrobial and antiadhesive activity, as well as increasing activity of NADP+-dependent glutamate dehydrogenase − a key enzyme of aminolipids biosynthesis. The minimum inhibitory concentration against Escherichia coli ІЕМ-1, Enterobacter cloaceae C-8, Staphylococcus aureus БМС-1 and Proteus vulgaris ПА-12 of surfactants, synthesized in the presence of Zn2+, and the adhesion of E. coli ІЕМ-1 on abiotic surfaces treated with such surfactants, were respectively in 1.6 - 3.3 times and 10 - 19 % lower than those of the preparations obtained under cultivation of ІMV B-7241 strain in medium without zinc cations. The activity of NADP+-dependent glutamate dehydrogenase at the end of exponential phase of A. calcoaceticus ІMV B-7241 growth in medium with ethanol (n-hexadecane), copper, zinc and iron sulfate, was 1739 ± 87 (8333 ± 416) nmol·min-1·mg-1 protein that in 2 and 15 times higher than under the same conditions cultivation on ethanol and n-hexadecane without Zn2+. Conclusions. The obtained data suggest the possibility of biosynthesis regulation of A. calcoaceticus ІMV B-7241 surfactants with antimicrobial and antiadhesive properties, when zinc cations (activator NADP+-dependent glutamate dehydrogenase − a key enzyme of aminolipids synthesis) were added into medium with ethanol (n-hexadecane), as well as the possibility of regulating the biological properties of the surfactants during cultivation of producer.
Key words: Acinetobacter calcoaceticus ІМВ В-7241, surfactants, zinc cations, activity of glutamate dehydrogenase, antimicrobial and antiadhesive activity.
Full text (PDF, in Russian)
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